Results 11 to 20 of about 515,050 (293)

CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

STAR Protocols, 2020
Summary: Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming ...
Yu Wang, Zhipeng Wang, Quanjiang Ji
doaj   +3 more sources

Editing base in mouse model [PDF]

Protein & Cell, 2017
Haoyi Wang
doaj   +3 more sources

Precision multiplexed base editing in human cells using Cas12a-derived base editors

Nature Communications
Base editors enable the direct conversion of target nucleotides without introducing DNA double strand breaks, making them a powerful tool for creating point mutations in a human genome. However, current Cas9-derived base editing technologies have limited
Anabel Y. Schweitzer, Etowah W. Adams, Michael T. A. Nguyen, Monkol Lek, Farren J. Isaacs   +4 more
doaj   +2 more sources

Applications of Base Editing Technology in Modifications of Pig Genes

Guangdong nongye kexue, 2022
Pigs are highly valued in both agriculture and medicine. We can quickly improve the economic properties of pigs and develop human disease models via gene modification technologies.
Jiasheng PAN, Qiuyan HUANG, Yecheng YANG, Shuaipeng YANG, Xiangxing ZHU, Dongsheng TANG   +5 more
doaj   +1 more source

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
doaj   +1 more source

Base and Prime Editing Technologies for Blood Disorders

Frontiers in Genome Editing, 2021
Nuclease-based genome editing strategies hold great promise for the treatment of blood disorders. However, a major drawback of these approaches is the generation of potentially harmful double strand breaks (DSBs).
Panagiotis Antoniou, Annarita Miccio, Mégane Brusson   +2 more
doaj   +1 more source

Frame-Based Editing [PDF]

Journal of Visual Languages and Sentient Systems, 2017
In introductory programming teaching, block-based editors have become very popular because they offer a number of strong advantages for beginning programmers: They avoid many syntax errors, can display all available instructions for visual selection and encourage experimentation with little requirement for recall. Among proficient programmers, however,
Kölling, Michael, Brown, Neil C. C., Altadmri, Amjad   +2 more
openaire   +1 more source

A streamlined base editor engineering strategy to reduce bystander editing

Nature Communications
Base editing (BE) can permanently correct over half of known human pathogenic genetic variants without requiring a repair template, thus serving as a promising therapeutic tool to treat a broad spectrum of genetic diseases.
Izabella Valdez, Ian O’Connor, Divesh Patel, Katherine Gierer, Jan Harrington, Ethan Ellis, Stephen A. Caponetti, Robert P. Sebra, Hillary C. Valley, Kevin Coote, Martin Mense, Samuele G. Marro, Tingting Jiang   +12 more
doaj   +2 more sources

Plant genome editing: CRISPR, base editing, prime editing, and beyond

Grassland Research, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system is a fast‐growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural ...
Yujie Xie, Syed Inzimam Ul Haq, Xingyu Jiang, Dianfeng Zheng, Naijie Feng, Wenying Wang, Jin‐Sheng He, Quan‐Sheng Qiu   +7 more
doaj   +1 more source

Non-viral in vivo cytidine base editing in hepatocytes using focused ultrasound targeted microbubbles

Molecular Therapy: Nucleic Acids, 2023
CRISPR-Cas9-based genome editing technologies, such as base editing, have the potential for clinical translation, but delivering nucleic acids into target cells in vivo is a major obstacle.
Cynthia D. Anderson, Jennifer Ataam Arthur, Yuan Zhang, Nike Bharucha, Ioannis Karakikes, Ralph V. Shohet   +5 more
doaj   +1 more source