Results 21 to 30 of about 4,580,472 (190)

Applications of Base Editing Technology in Modifications of Pig Genes

Guangdong nongye kexue, 2022
Pigs are highly valued in both agriculture and medicine. We can quickly improve the economic properties of pigs and develop human disease models via gene modification technologies.
Jiasheng PAN, Qiuyan HUANG, Yecheng YANG, Shuaipeng YANG, Xiangxing ZHU, Dongsheng TANG   +5 more
doaj   +1 more source

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
doaj   +1 more source

Base and Prime Editing Technologies for Blood Disorders

Frontiers in Genome Editing, 2021
Nuclease-based genome editing strategies hold great promise for the treatment of blood disorders. However, a major drawback of these approaches is the generation of potentially harmful double strand breaks (DSBs).
Panagiotis Antoniou, Annarita Miccio, Mégane Brusson   +2 more
doaj   +1 more source

Plant genome editing: CRISPR, base editing, prime editing, and beyond

Grassland Research, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system is a fast‐growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural ...
Yujie Xie, Syed Inzimam Ul Haq, Xingyu Jiang, Dianfeng Zheng, Naijie Feng, Wenying Wang, Jin‐Sheng He, Quan‐Sheng Qiu   +7 more
doaj   +1 more source

Non-viral in vivo cytidine base editing in hepatocytes using focused ultrasound targeted microbubbles

Molecular Therapy: Nucleic Acids, 2023
CRISPR-Cas9-based genome editing technologies, such as base editing, have the potential for clinical translation, but delivering nucleic acids into target cells in vivo is a major obstacle.
Cynthia D. Anderson, Jennifer Ataam Arthur, Yuan Zhang, Nike Bharucha, Ioannis Karakikes, Ralph V. Shohet   +5 more
doaj   +1 more source

PAM-expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G base editors for programmable base editing in mycobacteria [PDF]

, 2021
New therapeutic strategies for rapid and effective treatment of tuberculosis are highly desirable, and their development can be drastically accelerated by facile genetic manipulation methods in Mycobacterium tuberculosis.
Wei-Xiao, Wang, Xingxu, Huang, Quanjiang, Ji, Wei, Chen, Weizhong, Chen, Xia, Zhang, Hongyuan, Zhang, Yifei, Zhang   +7 more
core   +1 more source

Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer

Genomics, Proteomics & Bioinformatics, 2023
Base editing technology is being increasingly applied in genome engineering, but the current strategy for designing guide RNAs (gRNAs) relies substantially on empirical experience rather than a dependable and efficient in silico design.
Gongchen Zhang, Chenyu Zhu, Xiaohan Chen, Jifang Yan, Dongyu Xue, Zixuan Wei, Guohui Chuai, Qi Liu   +7 more
doaj   +1 more source

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Synthetic and Systems Biotechnology, 2020
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao   +5 more
doaj   +1 more source

Unbranched rod-like RNA is required for RNA editing of hepatitis delta virus genotype 2 and genotype 4

Virus Research, 2023
RNA editing of the hepatitis delta virus (HDV) is essential for generating the large delta antigen, which is crucial for virion assembly. In HDV genotype 1 (HDV-1), editing occurs within the context of the unbranched rod-like structure characteristic of ...
Chao-Wei Hsu, Hsueh-Ying Hsu, Chien-Hung Chen, Mei Chao   +3 more
doaj   +1 more source

Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors

Frontiers in Genome Editing, 2022
Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized
Cameron A. Burnett, Ashley T. Wong, Carlos A. Vasquez, Colleen A. McHugh, Gene W. Yeo, Gene W. Yeo, Alexis C. Komor   +6 more
doaj   +1 more source