Results 11 to 20 of about 19,262 (234)

Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Communications Biology, 2022
Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Fangbing Chen, Meng Lian, Bingxiu Ma, Shixue Gou, Xian Luo, Kaiming Yang, Hui Shi, Jingke Xie, Weika Ge, Zhen Ouyang, Chengdan Lai, Nan Li, Quanjun Zhang, Qin Jin, Yanhui Liang, Tao Chen, Jiaowei Wang, Xiaozhu Zhao, Lei Li, Manya Yu, Yinghua Ye, Kepin Wang, Han Wu, Liangxue Lai   +23 more
doaj   +3 more sources

A precise and efficient adenine base editor. [PDF]

Mol Ther, 2022
Adenine base editors (ABEs) are novel genome-editing tools, and their activity has been greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated catalytic activity was concomitant with frequent generation of bystander mutations. This bystander effect precludes its safe applications required in human gene therapy.
Tu T, Song Z, Liu X, Wang S, He X, Xi H, Wang J, Yan T, Chen H, Zhang Z, Lv X, Lv J, Huang XF, Zhao J, Lin CP, Gao C, Zhang J, Gu F.   +17 more
europepmc   +3 more sources

DNA capture by a CRISPR-Cas9-guided adenine base editor. [PDF]

Science, 2020
Secrets of a fast base editor CRISPR-Cas9 base editors comprise RNA-guided Cas proteins fused to an enzyme that can deaminate a DNA nucleoside. No natural enzyme deaminates adenine in DNA, and so a breakthrough came when a natural transfer RNA deaminase was fused to Cas9 and evolved to give an adenine base editor (
Lapinaite A, Knott GJ, Palumbo CM, Lin-Shiao E, Richter MF, Zhao KT, Beal PA, Liu DR, Doudna JA.   +8 more
europepmc   +8 more sources

Functional restoration of a CFTR splicing mutation through RNA delivery of CRISPR adenine base editor [PDF]

Molecular Therapy, 2023
Giulia Maule, Anna Cereseto
exaly   +2 more sources

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. [PDF]

Nat Biotechnol, 2023
AbstractCytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that retains the small size, low off-target activity and high on-target activity of current ABEs, we evolved the highly active deoxyadenosine deaminase TadA-8e to
Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR.   +13 more
europepmc   +3 more sources

Therapeutic adenine base editor with minimized off-target effects. [PDF]

Protein Cell
Abstract Genome-wide off-target effect poses a safety risk for clinical use of adenine base editor (ABE), among which ABE8e is one of the most efficient. Genome-wide off-target analysis by two-cell embryo injection (GOTI) analysis showed that the rate of genome-wide single-nucleotide variants (SNVs) in ABE8e-edited cells was ∼30-fold ...
Sun Y, Yan N, Feng H, Lu H, Zuo Z, Zhou C, Zuo E.   +6 more
europepmc   +3 more sources

Adenine base editor engineering reduces editing of bystander cytosines [PDF]

Nature Biotechnology, 2021
Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced ...
You Kyeong Jeong, SeokHoon Lee, Gue-Ho Hwang, Sung-Ah Hong, Se-eun Park, Jin-Soo Kim, Jae-Sung Woo, Sangsu Bae   +7 more
  +7 more sources

Optimize nearly PAM-less adenine base editor to improve editing efficiency [PDF]

Jichu yixue yu linchuang, 2022
Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e.
ZHOU Xiao-yu, WANG Xiao-yue
doaj   +1 more source

Optimizing plant adenine base editor systems by modifying the transgene selection system [PDF]

Plant Biotechnology Journal, 2020
Pengcheng Wei, Jianbo Yang, Xiuqing Zhang   +2 more
exaly   +2 more sources

Targeted A‐to‐T and A‐to‐C base replacement in maize using an optimized adenine base editor [PDF]

Plant Biotechnology Journal
Yuming Lu
exaly   +2 more sources