DNA capture by a CRISPR-Cas9–guided adenine base editor [PDF]
Science, 2020 Secrets of a fast base editor CRISPR-Cas9 base editors comprise RNA-guided Cas proteins fused to an enzyme that can deaminate a DNA nucleoside. No natural enzyme deaminates adenine in DNA, and so a breakthrough came when a natural transfer RNA deaminase was fused to Cas9 and evolved to give an adenine base editor (
Audronė Lapinaitė +8 more
europepmc +9 more sources
Genome-wide profiling of adenine base editor specificity by EndoV-seq [PDF]
Nature Communications, 2019 Adenine base editors are an important contribution to the genome editing toolbox. Here the authors present EndoV-seq, an endonuclease-based assay for evaluating genomewide off-target effects of base editing.
Puping Liang +11 more
doaj +5 more sources
Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos [PDF]
Commun Biol, 2020 AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study.
Hye‐Kyung Lee +4 more
europepmc +4 more sources
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation [PDF]
Molecular Therapy, 2020 Traditional CRISPR/Cas9-based gene knockouts are generated by introducing DNA double-strand breaks (DSBs), but this may cause excessive DNA damage or cell death. CRISPR-based cytosine base editors (CBEs) and adenine base editors (ABEs) can facilitate C-to-T or A-to-G exchanges, respectively, without DSBs.
Xinjie Wang, Lu Dang, Xingxu Huang
exaly +5 more sources
Optimizing plant adenine base editor systems by modifying the transgene selection system [PDF]
Plant Biotechnology Journal, 2020 Ruiying Qin +2 more
exaly +4 more sources
Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
Communications Biology, 2022 Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Fangbing Chen +23 more
doaj +3 more sources
A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9 [PDF]
BMC Biol, 2023 Yuqiang Qian +9 more
openalex +2 more sources
Adenine base editor–mediated correction of the common and severe IVS1-110 (G>A) β-thalassemia mutation [PDF]
Blood, 2022 Giulia Hardouin +10 more
openalex +2 more sources
A precise and efficient adenine base editor. [PDF]
Mol Ther, 2022 Adenine base editors (ABEs) are novel genome-editing tools, and their activity has been greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated catalytic activity was concomitant with frequent generation of bystander mutations. This bystander effect precludes its safe applications required in human gene therapy.
Tu T +17 more
europepmc +3 more sources
Adenine base editor‐based correction of the cardiac pathogenic Lmna c.
J Cell Mol MedLuzi Yang +5 more
openalex +2 more sources