Results 51 to 60 of about 19,445 (257)
Simplified adenine base editors improve adenine base editing efficiency in rice
Plant Biotechnology Journal, 2019 SummaryAdenine base editors (ABEs) have been exploited to introduce targeted adenine (A) to guanine (G) base conversions in various plant genomes, including rice, wheat and Arabidopsis. However, the ABEs reported thus far are all quite inefficient at many target sites in rice, which hampers their applications in plant genome engineering and crop ...
Kai Hua +5 more
openaire +2 more sources
TadA orthologs enable both cytosine and adenine editing of base editors
Nature Communications, 2023 AbstractCytidine and adenosine deaminases are required for cytosine and adenine editing of base editors respectively, and no single deaminase could enable concurrent and comparable cytosine and adenine editing. Additionally, distinct properties of cytidine and adenosine deaminases lead to various types of off-target effects, including Cas9-indendepent ...
Shuqian Zhang +9 more
openaire +3 more sources
Sequence-specific prediction of the efficiencies of adenine and cytosine base editors
Nature Biotechnology, 2020 Base editors, including adenine base editors (ABEs)1 and cytosine base editors (CBEs)2,3, are widely used to induce point mutations. However, determining whether a specific nucleotide in its genomic context can be edited requires time-consuming experiments.
Myungjae Song +14 more
openaire +3 more sources
Precise genome editing with base editors
Medical Review, 2023 Single-nucleotide variants account for about half of known pathogenic genetic variants in human. Genome editing strategies by reversing pathogenic point mutations with minimum side effects have great therapeutic potential and are now being actively ...
Liu Hongcai +3 more
doaj +1 more source
Discovery of novel disease-causing mutation in SSBP1 and its correction using adenine base editor to improve mitochondrial function. [PDF]
Mol Ther Nucleic AcidsMutations in nuclear genes regulating mitochondrial DNA (mtDNA) replication are associated with mtDNA depletion syndromes. Using whole-genome sequencing, we identified fi ed a heterozygous mutation (c.272G>A:p.Arg91Gln) in single-stranded DNA-binding ...
Cha JH +17 more
europepmc +2 more sources
Development of plant cytosine base editors with the Cas12a system
Crop Journal, 2023 Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs).
Huanhuan Wang +15 more
doaj +1 more source
Adenine Base Editing in an Adult Mouse Model of Tyrosinaemia
, 2022 Unlike traditional CRISPR-Cas9 homology-directed repair, base editing can correct point mutations without supplying a DNA-repair template. Here, we show in a mouse model of tyrosinemia that hydrodynamic tail-vein injection of plasmid DNA encoding the ...
Yin, Hao +14 more
core +1 more source
Communications Biology, 2021 Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells.
Jiao Fan +9 more
doaj +1 more source
Targeted Mutagenesis in Mice Using a Base Editor
, 2023 Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions.
Jeong, Tae Yeong +3 more
core +1 more source
In vivo adenine base editing corrects newborn murine model of Hurler syndrome
Molecular Biomedicine, 2023 Mucopolysaccharidosis type I (MPS I) is a severe disease caused by loss-of-function mutation variants in the α-L-iduronidase (Idua) gene. In vivo genome editing represents a promising strategy to correct Idua mutations, and has the potential to ...
Jing Su +10 more
doaj +1 more source