Results 51 to 60 of about 34,085 (286)
Rationalizing the Effect of Mutations on the Editing Efficiency of Adenine Base Editors [PDF]
Biophysical Journal, 2020 Kartik L. Rallapalli +2 more
openalex +2 more sources
Protocol for targeted modification of the rice genome using base editing
STAR Protocols, 2022 Summary: Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine ...
Meixia Wang, Fang Yan, Huanbin Zhou
doaj +1 more source
Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger [PDF]
Microb Cell FactGuoliang Yuan +4 more
openalex +2 more sources
Targeting fidelity of adenine and cytosine base editors in mouse embryos [PDF]
Nature Communications, 2018 AbstractBase editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits ...
Hye Kyung Lee +6 more
openaire +3 more sources
Therapeutic adenine base editing of human hematopoietic stem cells
Nature Communications, 2023 Here, Liao and colleagues apply adenine base editor ABE8e and its PAM-less variant ABE8e-SpRY to β-thalassemia patient hematopoietic stem cells in the form of ribonucleoprotein complexes, resulting in efficient long-term editing and β-thalassemia ...
Jiaoyang Liao +9 more
doaj +1 more source
Simplified adenine base editors improve adenine base editing efficiency in rice
Plant Biotechnology Journal, 2019 SummaryAdenine base editors (ABEs) have been exploited to introduce targeted adenine (A) to guanine (G) base conversions in various plant genomes, including rice, wheat and Arabidopsis. However, the ABEs reported thus far are all quite inefficient at many target sites in rice, which hampers their applications in plant genome engineering and crop ...
Kai Hua +5 more
openaire +2 more sources
Sequence-specific prediction of the efficiencies of adenine and cytosine base editors
Nature Biotechnology, 2020 Base editors, including adenine base editors (ABEs)1 and cytosine base editors (CBEs)2,3, are widely used to induce point mutations. However, determining whether a specific nucleotide in its genomic context can be edited requires time-consuming experiments.
Myungjae Song +14 more
openaire +3 more sources
Precise genome editing with base editors
Medical Review, 2023 Single-nucleotide variants account for about half of known pathogenic genetic variants in human. Genome editing strategies by reversing pathogenic point mutations with minimum side effects have great therapeutic potential and are now being actively ...
Liu Hongcai +3 more
doaj +1 more source
CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome
Rice Science, 2019 SUMMARY: Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A·T to G·C point mutation of
Li Hao +6 more
doaj +1 more source
Genomics and proteomics: a signal processor's tour [PDF]
, 2004 The theory and methods of signal processing are becoming increasingly important in molecular biology. Digital filtering techniques, transform domain methods, and Markov models have played important roles in gene identification, biological sequence ...
Vaidyanathan, P. P.
core +2 more sources