Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene [PDF]
, 2007 We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required.
Gidijala, Loknath, +3 more
core +2 more sources
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction [PDF]
Nature Biotechnology, 2018 Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) and adenine (ABE7.10) base editors by modification of nuclear localization signals (NLS) and codon usage, and ancestral reconstruction of the deaminase component.
Luke W Koblan +8 more
openaire +2 more sources
ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
BMC Biology, 2020 Background Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus.
Jingke Xie +20 more
doaj +1 more source
CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants
Molecular Therapy: Nucleic Acids, 2021 Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent ...
Wanyu Tao +10 more
doaj +1 more source
Non-mammalian model organisms in epigenetic research : an overview [PDF]
, 2020 Recent advances in sequencing technology and genome editing tools had an indisputably enormous impact on our understanding of complex biological pathways and their genetic and epigenetic regulation.
Baruah, Sri Kartik +3 more
core +2 more sources
Chirality in a quaternionic representation of the genetic code [PDF]
, 2015 A quaternionic representation of the genetic code, previously reported by the authors, is updated in order to incorporate chirality of nucleotide bases and amino acids.
Carlevaro, C. Manuel +2 more
core +2 more sources
Application of CRISPR-Cas System to Mitigate Superbug Infections
Microorganisms, 2023 Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within ...
Ali A. Rabaan +18 more
doaj +1 more source
Insights into DNA platination within unusual structural settings [PDF]
, 2011 2D [1H, 15N] HSQC NMR spectroscopy has been used to monitor reaction and product formation between [Pt(15NH3)2I2] and nucleic acids possessing irregular topologies and containing site specific phosphorothioate substitution in the phosphodiester backbone.
Harvie, Stephanie +2 more
core +2 more sources
Metabolic network percolation quantifies biosynthetic capabilities across the human oral microbiome [PDF]
, 2019 The biosynthetic capabilities of microbes underlie their growth and interactions, playing a prominent role in microbial community structure. For large, diverse microbial communities, prediction of these capabilities is limited by uncertainty about ...
Bernstein, David B. +2 more
core +1 more source
Human cells based directed evolution of adenine base editors with improved efficiency [PDF]
, 2021 Abstract Adenine base editors (ABE) are novel genome-editing tools that have been harnessed to introduce precise A•T to G•C conversion in genomic DNA. However, the low activity of ABE remains a major bottleneck that precludes efficacious applications.
Junhao Fu +9 more
openaire +1 more source