Results 91 to 100 of about 19,445 (257)

Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing

Journal of Extracellular Vesicles, 2021
Transient delivery of CRISPR‐based genome editing effectors is important to reduce off‐target effects and immune responses. Recently extracellular vesicles (EVs) have been explored for Cas9 ribonucleoprotein (RNP) delivery. However, lack of mechanisms to
Xingang Yao, Pin Lyu, Kyung Yoo, Manish Kumar Yadav, Ravi Singh, Anthony Atala, Baisong Lu   +6 more
doaj   +1 more source

Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

, 2023
Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells.
Haoyang Xiong, Tao Zhang, Wenchao Niu, Zhanjun Li, Ding Zhao, Mingyang Hu, Jinze Li, Di Wang, Xun Gao, Jiale Zhou, Yuqiang Qian, Liangxue Lai   +11 more
core  

Ligand‐Defined Interfacial Chemistry Enables Instant and Sequence‐General DNA Chemisorption on Gold Nanoparticles Toward Label‐Free SERS With Single‐Base Resolution

Advanced Science, EarlyView.
Ascorbic Acid‐Engineered Interface Enables Universal DNA Functionalization on Gold Nanoparticles: Attenuating electrostatic repulsion and leveraging synergistic hydrogen‐bonding interactions significantly lowers the kinetic barrier for DNA adsorption, enabling rapid and sequence‐general functionalization of non‐thiolated DNA‐requiring only a single ...
Guangping Li, Cheng Wang, Xinyue Wu, Zhongxiang Ding, Heng Gao, Zhonggang Liu, Yu Zhang, Jinai Chen, Feng Wang, Honglin Liu   +9 more
wiley   +1 more source

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

, 2023
Base editing requires that the target sequence satisfy the protospacer adjacent motif requirement of the Cas9 domain and that the target nucleotide be located within the editing window of the base editor.
Miller, Shannon, Fellmann, Christof, Huang, Tony, Oakes, Benjamin, Savage, David, Liu, David, Zhao, Kevin, Gaudelli, Nicole   +7 more
core   +1 more source

Heat Shock Protein 90: From Molecular Chaperone Function to Therapeutic Targeting in Malignancies

Advanced Science, EarlyView.
In this review, an integrated conceptual framework linking HSP90's molecular chaperone functions to its pathological roles in cancer is proposed. HSP90 serves as a central node that integrates oncogenic signaling, buffers proteotoxic stress, maintains cancer stem cell plasticity, and shapes tumor‐immune interactions, all of which converge to drive ...
Beibei Zhang, Xinxin Zou, Jiansong Zhou, Qinmiao Sun, Dahua Chen   +4 more
wiley   +1 more source

Adenine base editing efficiently restores the function of Fanconi anemia hematopoietic stem and progenitor cells

, 2022
Fanconi Anemia (FA) is a debilitating genetic disorder with a wide range of severe symptoms including bone marrow failure and predisposition to cancer.
Garcia-Garcia, Laura, Rio, Paula, Bueren, Juan A., Corn, Jacob E., Siegner, Sebastian M., Ugalde, Laura, Clemens, Alexandra, Corn, Jacob; id_orcid, Karasu, Mehmet E.   +8 more
core   +1 more source

Leonurine Ameliorates Doxorubicin‐Induced Cardiotoxicity via STING/NF‐κB/NLRP3 Inflammasome Signaling Pathway

Advanced Science, EarlyView.
ABSTRACT Doxorubicin‐induced cardiomyopathy (DIC) remains a dose‐limiting clinical challenge. This study reveals that cardiac vascular endothelial cells (CVECs) act as initial sensors of doxorubicin cardiotoxicity: circulating doxorubicin activates the cGAS‑STING pathway in CVECs, triggering NLRP3 inflammasome‑mediated pyroptosis and release of ...
Wang Jun, Chen Xiaoyang, Xu Jianglin, Yang Zhi, Tao Meijiao, He Jiale, Zhao Jiangfeng, Hei Xuanding, Xu Xuegong, Wang Wei, Li Chun   +10 more
wiley   +1 more source

Generation of the Camptothecin Scaffold by a Flavin‐Catalyzed Photooxidative Skeletal Reorganization

Angewandte Chemie, EarlyView.
The biosynthetic pathway of the anticancer drug precursor camptothecin involves an enigmatic skeletal reorganization from a 6/5/6 tetrahydro‐β‐carboline to a 6/6/5 pyrroloquinoline ring system. Here, we show that this transformation can be achieved by photooxidation with flavin cofactors, such as flavin mononucleotide (FMN) as catalysts.
Shenyu Liu, Zeliang Zhang, Benedikt Seligmann, Van Hung Bui, Tuan‐Anh Minh Nguyen, Thu‐Thuy T. Dang, Jakob Franke   +6 more
wiley   +2 more sources

Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses

, 2023
Base editors are widely used engineered proteins that introduce targeted point mutations into DNA without creating double-stranded DNA breaks. The application of base editors to study and treat genetic diseases is dependent on their in vivo delivery into
Liu, Qin, Davis, Jessie, Hennessey, Erin, Yeh, Wei Hsi, Pendse, Nachiket, Levy, Jonathan, Luke, Koblan, Liu, David, Butcher, Rossano, Comander, Jason   +9 more
core   +1 more source

Circulating Amino Acid Network Remodeling Reveals Systemic Metabolic Reprogramming Predictive of Colorectal Cancer Recurrence and Metastasis

Advanced Science, EarlyView.
Blood‐based amino acid patterns measured by 19F NMR reveal hidden metabolic changes in colorectal cancer. By analyzing how these amino acids interact as a network, machine learning models identify patients at higher risk of recurrence and metastasis.
Ji‐Yeon Lee, Jumi Kim, Taehan Yoon, So Hyun Kwon, Su Chan Park, Dohyun Chun, Dohyeong Kim, Eun Jung Park, Hyunwoo Kim, Ji Min Lee   +9 more
wiley   +1 more source