Results 81 to 90 of about 19,445 (257)

Genetic predisposition to porto‐sinusoidal vascular disorder: A functional genomic‐based, multigenerational family study

Hepatology, EarlyView., 2022
A deleterious variant of FCHSD1 results in mTOR pathway overactivation and may cause porto‐sinusoidal vascular disorder (PSVD). The pedigree of the family demonstrated an autosomal dominant disease with variable expressivity. Whole‐genome sequencing and Sanger sequencing both validated the existence of the FCHSD1 variant and the heterozygosity of c ...
Jingxuan Shan, André Megarbane, Aziz Chouchane, Deepak Karthik, Ramzi Temanni, Atilio Reyes Romero, Huiying Hua, Chun Pan, Xixi Chen, Murugan Subramanian, Chadi Saad, Hamdi Mbarek, Cybel Mehawej, Eliane Chouery, Sirin W. Abuaqel, Alexander Dömling, Sami Remadi, Cesar Yaghi, Pu Li, Lotfi Chouchane   +19 more
wiley   +1 more source

Base editing correction of hypertrophic cardiomyopathy in human cardiomyocytes and humanized mice

, 2023
reservedLa cardiomiopatia ipertrofica è una malattia cardiaca ereditaria causata da mutazioni dei geni che codificano proteine del sarcomero. È caratterizzata da un ispessimento del muscolo cardiaco e si manifesta con sintomi variabili, nei casi piu ...
MICHELOTTO, VITTORIA
core  

m6A‐Mediated Glycolysis by IL‐37 Drives T Cell Metabolic Reprogramming to Regulate Colitis

Advanced Science, EarlyView.
This study identifies an IL‐37/SIGIRR‐METTL14 regulatory axis that suppresses global m6A modification in CD4+ T cells. IL‐37 signaling, mediated through SIGIRR, inhibits IRAK4 and JNK phosphorylation, leading to downregulation of the methyltransferase METTL14.
Xiaoyan Wang, Jiadong Yu, Guolin Li, Ya Li, Fanlian Zeng, Jing Hu, Yifan Zhou, Chengcheng Yue, Qixiang Zhao, Chen Zhang, Hong Zhou, Fulei Zhao, Yawen Hu, Pei Zhou, Linna Gu, Yuting Feng, Mingxiang He, Shishi Huang, Xiaochi Sun, Xinmeng Wang, Jie Tang, Xinyu Zeng, Wenling Wu, Nongyu Huang, Xinai Cui, Kaijun Cui, Jiong Li   +26 more
wiley   +1 more source

The BnTFL1‐BnJAM3‐BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus

Advanced Science, EarlyView.
BnaC03.TFL1 and BnaA01.JAM3 form a complex that facilitates the transport of soluble sugars from the seed coat to the embryo by directly activating the expression of BnSWEETs. Furthermore, BnaC03.TFL1 and BnaA01.JAM3 work cooperatively to promote soluble sugar and oil production while suppressing protein deposition.
Jianjun Wang, Zijin Liu, Minshan Jin, Junting Liu, Shihao Wei, Dengmao Yang, Saiqi Yang, Yuan Guo, Mingxun Chen   +8 more
wiley   +1 more source

Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing

Cell Communication and Signaling, 2021
RNA base editing is potential for cellular function research and genetic diseases treating. There are two main RNA base editors, REPAIR and RESCUE, for in vitro use.
Guo Li, Yihan Wang, Xiangyang Li, Yuzhe Wang, Xingxu Huang, Jianen Gao, Xiaoxiang Hu   +6 more
doaj   +1 more source

Development of cytosine and adenine base editors for maize precision breeding

Journal of Integrative Plant Biology
ABSTRACTBase editing technologies can improve crops, but their efficiency in maize remains suboptimal. This study attempts to overcome these limitations by examining optimized cytosine and adenine base editors (CBEs and ABEs), namely evoAPOBEC1, evoFERNY, evoCDA1, TadA8.20, and TadA8e, for precise genome editing in transient and stable expression maize
Xiao Fu, Nan Wang, Lina Li, Dexin Qiao, Xiantao Qi, Changlin Liu, Zhaoxu Gao, Chuanxiao Xie, Jinjie Zhu   +8 more
openaire   +2 more sources

Improving adenine and dual base editors through introduction of TadA-8e and Rad51DBD

Nature Communications, 2023
AbstractBase editors, including dual base editors, are innovative techniques for efficient base conversions in genomic DNA. However, the low efficiency of A-to-G base conversion at positions proximal to the protospacer adjacent motif (PAM) and the A/C simultaneous conversion of the dual base editor hinder their broad applications.
Niannian Xue, Xu Liu, Dan Zhang, Youming Wu, Yi Zhong, Jinxin Wang, Wenjing Fan, Haixia Jiang, Biyun Zhu, Xiyu Ge, Rachel V. L. Gonzalez, Liang Chen, Shun Zhang, Peilu She, Zhilin Zhong, Jianjian Sun, Xi Chen, Liren Wang, Zhimin Gu, Ping Zhu, Mingyao Liu, Dali Li, Tao P. Zhong, Xiaohui Zhang   +23 more
openaire   +3 more sources

DMS‐MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity

Advanced Science, EarlyView.
RNA structural profiling of Turnip Yellow Mosaic Virus by DMS‐MaPseq and DREEM analyses uncover that viral genome‐wide RSS is highly complicated and heterogeneous, with alternative RSSs widely distributed across the genome. Notably, the viral 3’ tRNA‐like structure adopts alternative conformations in vivo.
Jiaying Zhu, Changhao Li, Chisimbily Onyia, Xindi Li, Xingxing Yan, Songxiao Zhong, Taerin Oh, Xiuren Zhang   +7 more
wiley   +1 more source

Research progress of base editing and prime editing tools based on the CRISPR/Cas system

Molecular Therapy: Nucleic Acids
The base editor (BE) and prime editing guide RNA (pegRNA)-based prime editor (PE) technologies relying on the CRISPR/Cas system are very efficient gene editors that have been developed in recent years.
Herui Gao, Song Gao, Guoshi Kan, Leonid N. Valentovich, Yingfeng An   +4 more
doaj   +1 more source

Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease

Frontiers in Cell and Developmental Biology, 2020
Human pluripotent stem cells (hPSCs) are a powerful platform for disease modeling and drug discovery. However, the introduction of known pathogenic mutations into hPSCs is a time-consuming and labor-intensive process.
Tao Qi, Fujian Wu, Yuquan Xie, Siqi Gao, Miaomiao Li, Jun Pu, Dali Li, Feng Lan, Yongming Wang, Yongming Wang   +9 more
doaj   +1 more source