Results 11 to 20 of about 63,943 (301)

CoCl₂-Mimicked Endothelial Cell Hypoxia Induces Nucleotide Depletion and Functional Impairment That Is Reversed by Nucleotide Precursors

Biomedicines, 2022
Chronic hypoxia drives vascular dysfunction by various mechanisms, including changes in mitochondrial respiration. Although endothelial cells (ECs) rely predominantly on glycolysis, hypoxia is known to alter oxidative phosphorylation, promote oxidative ...
Barbara Kutryb-Zajac, Ada Kawecka, Alicja Braczko, Marika Franczak, Ewa M. Slominska, Roberto Giovannoni, Ryszard T. Smolenski   +6 more
doaj   +1 more source

A liquid chromatography-tandem mass spectrometry based method for the quantification of adenosine nucleotides and NAD precursors and products in various biological samples

Frontiers in Immunology, 2023
Adenine nucleotides (AN) are ubiquitous metabolites that regulate cellular energy metabolism and modulate cell communication and inflammation. To understand how disturbances in AN balance arise and affect cellular function, robust quantification ...
Johanna Hiefner, Johann Rische, Madeleine J. Bunders, Madeleine J. Bunders, Madeleine J. Bunders, Anna Worthmann   +5 more
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Insight into an Oxidative DNA-Cleaving DNAzyme: Multiple Cofactors, the Catalytic Core Map and a Highly Efficient Variant

iScience, 2020
Summary: An oxidative DNA-cleaving DNAzyme (PL) employs a double-cofactor model “X/Cu2+” for catalysis. Herein, we verified that reduced nicotinamide adenine dinucleotide (NADH), flavin mononucleotide, cysteine, dithiothreitol, catechol, resorcinol ...
Wenqian Yu, Shijin Wang, Dongling Cao, Hongyue Rui, Chengcheng Liu, Yongjie Sheng, Yanhong Sun, Jin Zhang, Jiacui Xu, Dazhi Jiang   +9 more
doaj   +1 more source

Substrate binding in the mitochondrial ADP/ATP carrier is a step-wise process guiding the structural changes in the transport cycle

Nature Communications, 2022
The mitochondrial ADP/ATP carrier transports adenine nucleotides. Here, authors identify the residues involved in substrate binding. One set forms the central substrate binding site and two asparagine/arginine pairs help to guide the substrates during ...
Vasiliki Mavridou, Martin S. King, Sotiria Tavoulari, Jonathan J. Ruprecht, Shane M. Palmer, Edmund R. S. Kunji   +5 more
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Intracellular ATP influences synaptic plasticity in area CA1 of rat hippocampus via metabolism to adenosine and activity-dependent activation of adenosine A1 receptors [PDF]

, 2011
The extent to which brain slices reflect the energetic status of the in vivo brain has been a subject of debate. We addressed this issue to investigate the recovery of energetic parameters and adenine nucleotides in rat hippocampal slices and the ...
D. Grahame Hardie, Hardie, D. G. (D. Grahame), Naomi Pentland, Simon Hawley, Frenguelli, Bruno, Doney, Alexander S., Pentland, Naomi, Frenguelli, Bruno G., Stephanie zur Nedden, Hawley, Simon, Alexander S. Doney, Bruno G. Frenguelli, Hardie, D. Grahame, Doney, Alexander S.; id_orcid, Zur Nedden, Stephanie   +14 more
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Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants [PDF]

Current Protocols in Plant Biology, 2020
AbstractAs the principal co‐factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their rapid and reversible conversion as well as their relatively low concentration ranges, it is difficult to measure these ...
Zhang, Youjun, Krahnert, Ina, Bolze, Antje, Gibon, Yves, Fernie, Alisdair   +4 more
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The evolution of purinergic receptors involved in recognition of a blood meal by hematophagous insects

Memorias do Instituto Oswaldo Cruz, 1987
Many blood feeders use adenine nucleotides as cues for locating blood meal. Structure-activity relationship of adenine nucleotides as phagostimulants varies between closely-related species of blood feeders. It is suggested that a preexisting diverse pool
Rachel Galun
doaj   +1 more source

Development and application of LC/HRPS for quantification of adenine nucleotides, creatine phosphate, and creatine in sturgeon spermatozoa

Czech Journal of Animal Science, 2017
The objective of this study was to investigate the applicability of liquid chromatography coupled with high resolution product scan (LC/HRPS) mass spectrometry for simultaneous quantification of adenine nucleotides, creatine phosphate, and creatine ...
Pavlo Fedorov, Roman Grabic, Ganna Fedorova, Jacky Cosson, Sergii Boryshpolets, Borys Dzyuba   +5 more
doaj   +1 more source

Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC [PDF]

, 2012
Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways.
Claire M. Peppiatt-Wildman, Scott-Ward, Toby S., Alberto Contreras-Sanz, Kevin M. G. Taylor, Malone-Lee, James, Wildman, Scott S.P., Rebecca E. Birch, Scott S. P. Wildman, James Malone-Lee, Jennifer C. Jacoby, Taylor, Kevin M. G., Gill, Hardyal S., Peppiatt-Wildman, Claire M., Birch, Rebecca E., Contreras-Sanz, Alberto, Hardyal S. Gill, Toby S. Scott-Ward, Jacoby, Jennifer C., Wildman, Scott S. P.   +18 more
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Conformationally Restricted Adenine Nucleotide Analogs in Mitochondrial Adenine Nucleotide Transport

Zeitschrift für Naturforschung C, 1978
Abstract The conformationally restricted adenine nucleotide analogs 8,3′anhydro-8-oxy-9-(β-ᴅ-xylofurano-syl) adenine-5′-O-tri (di) -phosphate (I) , and 8,2′-anhydro-8-oxy-9-(β-ᴅ-arabinofuranosyl) adenine-5′-O-tri (di)-phosphate (II), were prepared chemically as their α- 32 P-
K S, Boos, E, Schlimme, M, Ikehara
openaire   +2 more sources