Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
Advanced Science, 2019 Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical ...
Dan Wang +9 more
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Replication of an adeno-associated virus in canine and human cells with infectious canine hepatitis virus as a "helper" [PDF]
, 1967 Kendall O. Smith, Warren D. Gehle
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Advanced Science, EarlyView.This study uncovers CHKA as a pivotal driver of vascular dysfunction in diabetic retinopathy and highlights its role in endothelial dysfunction through NAD⁺‐SIRT1‐Notch signaling. Single‐cell and functional analyses, combined with clinical and genetic validation, suggest CHKA as a promising therapeutic target for the prevention and treatment of ...
Ling Ren +11 more
wiley +1 more source
Retardation of atherosclerosis in immunocompetent apolipoprotein (apo) E-deficient mice followingliver-directed administration of a [E1-, E3-,polymerase-] adenovirus vector containing the elongation factor-1a promoter driving expression of human apoE cDNA [PDF]
, 2005 Although gene transfer of human apolipoprotein E (apoE), a 34-kDa circulating glycoprotein, to the liver of apoEdeficient(apoE-/-) mice using recombinant adenoviral vectors (rAd) is antiatherogenic, its full therapeutic potentialhas yet to be realized ...
Amalfitano, A +6 more
core
Size does matter: overcoming the adeno-associated virus packaging limit
Respiratory Research, 2000 Recombinant adeno-associated virus (rAAV) vectors mediate long-term gene transfer without any known toxicity. The primary limitation of rAAV has been the small size of the virion (20 nm), which only permits the packaging of 4.7 kilobases (kb) of ...
Flotte Terence R
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Nucleic acid from an adeno-associated virus: chemical and physical studies. [PDF]
, 1966 J. A. Rose, M D Hoggan, Aaron J. Shatkin
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Molecular design for recombinant adeno-associated virus (rAAV) vector production
Applied Microbiology and Biotechnology, 2017 Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long ...
Juan Jose Aponte-Ubillus +5 more
semanticscholar +1 more source
Advanced Science, EarlyView.This study investigates the role of macrophage pyruvate carboxylase (PC) in atherosclerosis (AS) demonstrating that PC upregulation in macrophages promotes metabolism reprogramming to enhance inflammatory responses via the HIF‐1 signaling pathway.
Ling‐Na Zhao +17 more
wiley +1 more source
Adenovirus E1A Activation Domain Regulates H3 Acetylation Affecting Varied Steps in Transcription at Different Viral Promoters. [PDF]
, 2018 How histone acetylation promotes transcription is not clearly understood. Here, we confirm an interaction between p300 and the adenovirus 2 large E1A activation domain (AD) and map the interacting regions in E1A by observing colocalization at an ...
Berk, Arnold J +4 more
core
Vector design influences hepatic genotoxicity after adeno-associated virus gene therapy.
Journal of Clinical Investigation, 2015 The use of adeno-associated virus (AAV) as a gene therapy vector has been approved recently for clinical use and has demonstrated efficacy in a growing number of clinical trials.
R. Chandler +10 more
semanticscholar +1 more source