Results 181 to 190 of about 74,668 (263)

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Liver-specific gene therapy based on self-complementary adeno-associated virus for lysosomal acid lipase deficiency. [PDF]

Front Pharmacol
Zhang R, Lou J, Jin Z, Ou Y, He T, Qu H, Yang Y, Qu X.   +7 more
europepmc   +1 more source

Enhancing Adeno‐Associated Virus 2 Capsid Engineering Through Co‐Evolutionary Coupling

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated virus (AAV) has emerged as a leading platform for gene therapy. However, to unlock the full potential, their manufacturing yields, stability, and efficacy must all be improved. Rational design is limited by poor predictability and the potential impact of mutations on multiple important vector properties.
Sirimar Laosinwattana, Yiwen Li, Braulio Carrillo Sanchez, Ayesha Khan, Paul A. Dalby   +4 more
wiley   +1 more source

Lyophilization of Adeno-Associated Virus Serotypes for Storage and Global Distribution. [PDF]

Biomedicines
McGlinch EB, Mudrick HE, Evans CH, Barry MA.   +3 more
europepmc   +1 more source

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Hemophilia B gene therapy: a NAb-solute barrier overcome by adeno-associated virus serotype 5. [PDF]

Res Pract Thromb Haemost
Kaczmarek R.
europepmc   +1 more source

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe, Joseph D. Barritt, Nichakorn Pipatpadungsin, Braulio Carrillo Sanchez, Dale Stibbs, Lisa Cooper, Sarah L. Rouse, Daniel G. Bracewell   +7 more
wiley   +1 more source

Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system. [PDF]

Mol Ther Adv
Joseph J, Destro F, Yuan A, Antov D, Chen W, Song S, Theriault M, Yuan C, Sansom A, Lundin C, Burns T, Hamed HA, Morales P, Fan JX, Rodriguez AR, Sichuma S, McCracken C, Neufeld C, Wolfrum JM, Srinivasan P, Barone PW, Sinskey AJ, Braatz RD, Springs SL.   +23 more
europepmc   +1 more source

Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modified Vaccinia virus Ankara (MVA) is a potent vector for the development of vaccines and novel cancer treatment strategies. Since it is a non‐replicating virus in human recipients, high infective titers are required for therapeutic efficacy.
Linus G. Weber, Caroline Stephan, Georgios Makris, Leander John, Ingo Jordan, Michael W. Wolff   +5 more
wiley   +1 more source