Results 121 to 130 of about 227,189 (359)

Solution‐Based Biophysical Methods for Guiding Design of Aptamers into Electrochemical Biosensors

open access: yesAnalysis &Sensing, EarlyView.
It is found that aptamer binding and folding thermodynamics measured via isothermal titration calorimetry and nano differential scanning calorimetry directly correlate with electrochemical aptamer‐based biosensor response. We show that these techniques report on the magnitude of conformational change and could be used as guides to help engineer ...
Minh‐Dat Nguyen   +4 more
wiley   +1 more source

Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK). [PDF]

open access: yes, 1979
Madin-Darby canine kidney (MDCK) cells grown in tissue culture have the morphological properties of distal tubular epithelial cells, form tight junctions, and lack several proximal tubular enzyme markers.
Chuman, LM   +3 more
core  

Fresh insights into the light‐induced pineal gland circadian rhythm transmission mechanism derived from mRNA and miRNA profiling

open access: yesAnimal Research and One Health, EarlyView.
Mechanisms of light in regulating the chick pineal clock. Abstract The circadian clock significantly impacts animal health and productivity, with light playing a crucial role in regulating circadian rhythms. However, the mechanisms behind light‐induced circadian transmission remain unclear, particularly in light‐sensitive avian species.
Yunlei Li   +10 more
wiley   +1 more source

ThiF‐Like Enzyme Chemistry in Primary and Secondary Metabolism

open access: yesChemBioChem, EarlyView.
ThiF‐like enzymes are a widespread protein family found in disparate biosynthetic pathways. They are unified by their use of an NTP to modify a carboxylate, generating an activated species prone to nucleophilic addition. This common intermediate is then targeted by diverse nucleophiles, including persulfide or amino acid side chains, to yield a variety
Keelie S. Butler   +2 more
wiley   +1 more source

Development and Validation of a High‐Throughput Screening Assay for the Legionella ADP‐Ribosyl Transferase SdeA

open access: yesChemBioChem, EarlyView.
We show the optimization and application of a fluorogenic assay to monitor Legionella SdeA activity in a High‐Throughput‐Screening campaign. Ubiquitination of proteins is one of the most crucial post‐translational modifications in eukaryotic cells, typically involving conjugation of ubiquitin to a lysine residue in a substrate using a three‐enzyme ...
Halana C. Vlaming   +6 more
wiley   +1 more source

Citrus Flavonoids for Cystic Fibrosis Treatment

open access: yesChemBioChem, EarlyView.
Studies conducted since the late 1990s show substantial activity of selected citrus flavonoids as cystic fibrosis transmembrane conductance regulator (CFTR) membrane protein activators. The thesis of this work is that aptly formulated citrus flavonoids hold sigificant potential for developing a multitarget treatment of cystic fibrosis combining in a ...
Mario Pagliaro   +5 more
wiley   +1 more source

CAQ Corner: Basic concepts of transplant immunology

open access: yes, 2022
Liver Transplantation, EarlyView.
Amanda Cheung, Josh Levitsky
wiley   +1 more source

Effect of Specific Spoilage Organisms on the Degradation of ATP-Related Compounds in Vacuum-Packed Refrigerated Large Yellow Croaker (Larimichthys crocea)

open access: yesFoods
This study examined the spoilage potential of specific spoilage organisms on the degradation of adenosine triphosphate (ATP)-related compounds in vacuum-packed refrigerated large yellow croaker.
Bohan Chen   +4 more
doaj   +1 more source

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