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Dexras1 inhibits adenylyl cyclase

Biochemical and Biophysical Research Communications, 2004
Dexras1 is a steroid hormone-induced Ras family G protein that acts as a receptor-independent activator of signaling by Gi/o family heterotrimeric G proteins. We examined the effects of Dexras1 on the activity of adenylyl cylase, a target of inhibitory regulation by Gialpha x GTP.
T.E. Graham, Z. Qiao, R.I. Dorin
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Conversion of a Guanylyl Cyclase to an Adenylyl Cyclase

Methods, 1999
Guanylyl cyclases catalyze the formation of cGMP from GTP, but display extensive identity at the catalytic domain primary amino acid level with the adenylyl cyclases. The recent solving of the crystal structures of soluble forms of adenylyl cyclase has resulted in predictions of those amino acids important for substrate specificity.
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Adenylyl and Guanylyl Cyclase Assays

Current Protocols in Pharmacology, 2005
AbstractThis unit presents two basic protocols to determine adenylyl cyclase and guanylyl cyclase activity in tissue and cell homogenates, permeabilized cells, or subcellular fractions. Each method is divided into two parts: the enzyme reaction that causes the formation of the labeled cyclic nucleotide, and the separation of cyclic nucleotide products ...
Troy Stevens   +3 more
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Adenylyl Cyclases

2010
Publisher Summary This chapter presents the structure and catalytic mechanism of adenylyl cyclase (AC) and focuses on many modes of AC regulation. It also discusses normal and aberrant AC function in physiology and disease states. Hormonal and neurotransmitter regulation of AC occurs primarily through heterotrimeric G proteins in both vertebrates and ...
Roger K. Sunahara, Adam J. Kuszak
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The adenylyl and guanylyl cyclase superfamily

Current Opinion in Structural Biology, 1998
New structures solved in 1997 revealed that the adenylyl cyclase core consists of a pair of catalytic domains arranged in a wreath. Homologous catalytic domains are arranged in diverse adenylyl and guanylyl cyclases as symmetric homodimers or pseudosymmetric heterodimers.
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Photoaffinity Labeling of Adenylyl Cyclase

2002
Photoaffinity is an effective tool to covalently tag amino acid residues that are in or near ligand-binding sites. The polypeptide chains that contribute to the binding site of a given receptor can be identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) if they are specifically labeled with a radioactive marker ...
Gülhan Pilli   +5 more
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Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor.

Science, 2000
Spermatozoa undergo a poorly understood activation process induced by bicarbonate and mediated by cyclic adenosine 3',5'-monophosphate (cAMP). It has been assumed that bicarbonate mediates its effects through changes in intracellular pH or membrane ...
Yanqiu Chen   +6 more
semanticscholar   +1 more source

Purification of Soluble Adenylyl Cyclase

2002
Cyclic AMP (cAMP), the nearly universal second messenger, is synthesized by a broad family of adenylyl cyclases (ACs). Mammals have at least nine isoforms of transmembrane-spanning adenylyl cyclases (tmACs). These differ in their expression patterns, and their catalytic activities are differentially regulated by G proteins and other signaling molecules.
Jochen Buck   +2 more
openaire   +3 more sources

Characterization and engineering of photoactivated adenylyl cyclases

Biological Chemistry, 2019
Abstract Cyclic nucleoside monophosphates (cNMP) serve as universal second messengers in signal transduction across prokaryotes and eukaryotes. As signaling often relies on transiently formed microdomains of elevated second messenger concentration, means to precisely perturb the spatiotemporal dynamics of cNMPs are uniquely poised for ...
Birthe Stüven   +5 more
openaire   +4 more sources

Type-specific regulation of adenylyl cyclase by G protein beta gamma subunits.

Science, 1991
Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) dissociate into guanosine triphosphate (GTP)-bound alpha subunits and a complex of beta and gamma subunits after interaction with receptors.
Wei-Jen Tang, A. Gilman
semanticscholar   +1 more source

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