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Conversion of a Guanylyl Cyclase to an Adenylyl Cyclase
Methods, 1999Guanylyl cyclases catalyze the formation of cGMP from GTP, but display extensive identity at the catalytic domain primary amino acid level with the adenylyl cyclases. The recent solving of the crystal structures of soluble forms of adenylyl cyclase has resulted in predictions of those amino acids important for substrate specificity.
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Dexras1 inhibits adenylyl cyclase
Biochemical and Biophysical Research Communications, 2004Dexras1 is a steroid hormone-induced Ras family G protein that acts as a receptor-independent activator of signaling by Gi/o family heterotrimeric G proteins. We examined the effects of Dexras1 on the activity of adenylyl cylase, a target of inhibitory regulation by Gialpha x GTP.
T E, Graham, Z, Qiao, R I, Dorin
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Adenylyl and Guanylyl Cyclase Assays
Current Protocols in Pharmacology, 2005AbstractThis unit presents two basic protocols to determine adenylyl cyclase and guanylyl cyclase activity in tissue and cell homogenates, permeabilized cells, or subcellular fractions. Each method is divided into two parts: the enzyme reaction that causes the formation of the labeled cyclic nucleotide, and the separation of cyclic nucleotide products ...
John J, Kelly +3 more
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The adenylyl and guanylyl cyclase superfamily
Current Opinion in Structural Biology, 1998New structures solved in 1997 revealed that the adenylyl cyclase core consists of a pair of catalytic domains arranged in a wreath. Homologous catalytic domains are arranged in diverse adenylyl and guanylyl cyclases as symmetric homodimers or pseudosymmetric heterodimers.
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Purification of Soluble Adenylyl Cyclase
2002Cyclic AMP (cAMP), the nearly universal second messenger, is synthesized by a broad family of adenylyl cyclases (ACs). Mammals have at least nine isoforms of transmembrane-spanning adenylyl cyclases (tmACs). These differ in their expression patterns, and their catalytic activities are differentially regulated by G proteins and other signaling molecules.
Jochen, Buck +2 more
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Photoaffinity Labeling of Adenylyl Cyclase
2002Photoaffinity is an effective tool to covalently tag amino acid residues that are in or near ligand-binding sites. The polypeptide chains that contribute to the binding site of a given receptor can be identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) if they are specifically labeled with a radioactive marker ...
Michael K, Sievert +5 more
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