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Adenylyl and Guanylyl Cyclase Assays
Current Protocols in Pharmacology, 2005AbstractThis unit presents two basic protocols to determine adenylyl cyclase and guanylyl cyclase activity in tissue and cell homogenates, permeabilized cells, or subcellular fractions. Each method is divided into two parts: the enzyme reaction that causes the formation of the labeled cyclic nucleotide, and the separation of cyclic nucleotide products ...
Troy Stevens +3 more
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2010
Publisher Summary This chapter presents the structure and catalytic mechanism of adenylyl cyclase (AC) and focuses on many modes of AC regulation. It also discusses normal and aberrant AC function in physiology and disease states. Hormonal and neurotransmitter regulation of AC occurs primarily through heterotrimeric G proteins in both vertebrates and ...
Roger K. Sunahara, Adam J. Kuszak
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Publisher Summary This chapter presents the structure and catalytic mechanism of adenylyl cyclase (AC) and focuses on many modes of AC regulation. It also discusses normal and aberrant AC function in physiology and disease states. Hormonal and neurotransmitter regulation of AC occurs primarily through heterotrimeric G proteins in both vertebrates and ...
Roger K. Sunahara, Adam J. Kuszak
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The adenylyl and guanylyl cyclase superfamily
Current Opinion in Structural Biology, 1998New structures solved in 1997 revealed that the adenylyl cyclase core consists of a pair of catalytic domains arranged in a wreath. Homologous catalytic domains are arranged in diverse adenylyl and guanylyl cyclases as symmetric homodimers or pseudosymmetric heterodimers.
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Photoaffinity Labeling of Adenylyl Cyclase
2002Photoaffinity is an effective tool to covalently tag amino acid residues that are in or near ligand-binding sites. The polypeptide chains that contribute to the binding site of a given receptor can be identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) if they are specifically labeled with a radioactive marker ...
Gülhan Pilli +5 more
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Purification of Soluble Adenylyl Cyclase
2002Cyclic AMP (cAMP), the nearly universal second messenger, is synthesized by a broad family of adenylyl cyclases (ACs). Mammals have at least nine isoforms of transmembrane-spanning adenylyl cyclases (tmACs). These differ in their expression patterns, and their catalytic activities are differentially regulated by G proteins and other signaling molecules.
Jochen Buck +2 more
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Characterization and engineering of photoactivated adenylyl cyclases
Biological Chemistry, 2019Abstract Cyclic nucleoside monophosphates (cNMP) serve as universal second messengers in signal transduction across prokaryotes and eukaryotes. As signaling often relies on transiently formed microdomains of elevated second messenger concentration, means to precisely perturb the spatiotemporal dynamics of cNMPs are uniquely poised for ...
Birthe Stüven +5 more
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Regulation of forskolin interactions with type I, II, V, and VI adenylyl cyclases by Gs alpha.
Biochemistry, 1994Several forms of adenylyl cyclase (types I, II, V, and VI) have been expressed using the recombinant baculovirus expression system in Sf9 cells. The activation of type I adenylyl cyclase by forskolin and Gs alpha was not greater than additive.
Elizabeth McHugh Sutkowski +4 more
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Endocrinology, 1985
Membrane-bound adenylyl cyclases from ram, dog, and human sperm are unresponsive to fluoride and guanylylimidodiphosphate [GMP-P(NH)P], two agents that stimulate the adenylyl cyclases of somatic cells by an action on the stimulatory guanine nucleotide ...
J. Hildebrandt +6 more
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Membrane-bound adenylyl cyclases from ram, dog, and human sperm are unresponsive to fluoride and guanylylimidodiphosphate [GMP-P(NH)P], two agents that stimulate the adenylyl cyclases of somatic cells by an action on the stimulatory guanine nucleotide ...
J. Hildebrandt +6 more
semanticscholar +1 more source
Tyrphostins Are Inhibitors of Guanylyl and Adenylyl Cyclases
Biochemistry, 2004Guanylyl cyclase C (GC-C), the receptor for guanylin, uroguanylin, and the heat-stable enterotoxin, regulates fluid balance in the intestine and extraintestinal tissues. The receptor has an extracellular domain, a single transmembrane spanning domain, and an intracellular domain that harbors a region homologous to protein kinases, followed by the C ...
Jaleel, Mahaboobi +2 more
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Regulation of adenylyl cyclase in LTP
Behavioral and Brain Sciences, 1995AbstractOur results on hippocampal long-term potentiation are considered in the context of Xia et al.'s hypothesis. Whereas the target article proposes presynaptic PKC involvement in adenylyl cyclase activation by phosphorylation of nenromodulin, we suggest an additional postsynaptic role involving RC3/nenrogranin.
Erik D. Roberson, J. David Sweatt
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