Results 101 to 110 of about 2,157,684 (307)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Antibodies Are Not Essential for the Resolution of Primary Cytomegalovirus Infection but Limit Dissemination of Recurrent Virus [PDF]
, 1994 Virus shedding from the epithelial cells of the serous acini of salivary glands is a major source for the horizontal transmission of cytomegalovirus. These cells are, different to other tissues, exempt from CD8 T lymphocyte control. CD4 T lymphocytes are
Crnkovic, Irena +5 more
core
, 2010 Glioblastoma multiforme (GBM), which occurs mostly in individuals over the age of 40, accounts for 12–15% of all primary brain tumors. Patients with GBM have a poor prognosis, even after aggressive upfront therapies. The present study documents that in 5
Yoshiaki Fujimiya +4 more
core +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Improving TCR Gene Therapy for Treatment of Haematological Malignancies
Advances in Hematology, 2012 Adoptive immunotherapy using TCR gene modified T cells may allow separation of beneficial Graft versus tumour responses from harmful GvHD. Improvements to this include methods to generate high avidity or high affinity TCR, improvements in vector design ...
Emma Nicholson +2 more
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Adoptive transfer of immunity to hepatitis B virus by liver transplantation in rats [PDF]
, 2002 We used a rat model to study adoptive immune transfer by liver transplantation. Animals' humoral response to HBsAg was chosen as the parameter to judge the efficacy of the immune transfer.
Li, Jun
core
, 1998 ESb lymphoma cells injected i,v, into DBA/2 (H-2(d)) mice multiply rapidly in the liver and kill ail mice in a few days. Adoptive transfer of allogeneic C57Bl/6 (H-2(b)) tumor-immune or normal splenic lymphocytes to sublethally irradiated DBA/2 mice ...
VENDITTI, Mario +9 more
core +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Characterization of a transgenic mouse model of chronic conditional platelet depletion
Research and Practice in Thrombosis and Haemostasis, 2019 Background Platelets are widely recognized for their role in maintaining hemostasis. Recently, platelets have become appreciated for their varying roles in immunity, neuroprotection, and other physiological processes.
Leah M. Wuescher +2 more
doaj +1 more source