Characterization of a Chinese hamster ovary cell mutant having a mutation in elongation factor-2. [PDF]
PLoS ONE, 2010 Retroviral insertional mutagenesis provides an effective forward genetic method for identifying genes involved in essential cellular pathways. A Chinese hamster ovary cell line mutant resistant to several bacterial ADP-ribosylating was obtained by this ...
Pradeep K Gupta +2 more
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mBio, 2016 ADP-ribosylation is a common posttranslational modification that may have antiviral properties and impact innate immunity. To regulate this activity, macrodomain proteins enzymatically remove covalently attached ADP-ribose from protein targets.
Anthony R. Fehr +8 more
doaj +1 more source
Inhibition of Cell Migration by PITENINs: The Role of ARF6 [PDF]
, 2012 We have previously reported the development of small molecule phosphatidylinositol-3,4,5-trisphosphate (PIP3) antagonists (PITs) that block pleckstrin homology (PH) domain interaction, including activation of Akt, and show anti-tumor potential.
Degterev, Alexei +9 more
core +1 more source
ADP-Ribosylation and Antiviral Resistance in Plants
Viruses, 2023 ADP-ribosylation (ADPRylation) is a versatile posttranslational modification in eukaryotic cells which is involved in the regulation of a wide range of key biological processes, including DNA repair, cell signalling, programmed cell death, growth and ...
Nadezhda Spechenkova +4 more
doaj +1 more source
C9orf72 Toxic Species Affect ArfGAP-1 Function
Cells, 2023 Compelling evidence indicates that defects in nucleocytoplasmic transport contribute to the pathogenesis of amyotrophic lateral sclerosis (ALS). In particular, hexanucleotide (G4C2) repeat expansions in C9orf72, the most common cause of genetic ALS, have
Simona Rossi +9 more
doaj +1 more source
A target repurposing approach identifies N-myristoyltransferase as a new candidate drug target in filarial nematodes [PDF]
, 2014 Myristoylation is a lipid modification involving the addition of a 14-carbon unsaturated fatty acid, myristic acid, to the N-terminal glycine of a subset of proteins, a modification that promotes their binding to cell membranes for varied biological ...
Carlow, Clotilde K. S. +7 more
core +4 more sources
PARP10 is critical for stress granule initiation
Life Science AllianceThis study identifies PARP10 as a key regulator of stress granule assembly by ADP-ribosylating G3BP1 and modulating eIF2α phosphorylation and translation factor recruitment. Stress granules (SGs) are cytoplasmic biomolecular condensates enriched with RNA
Aravinth Kumar Jayabalan +2 more
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Coordinated actions of microRNAs with other epigenetic factors regulate skeletal muscle development and adaptation [PDF]
, 2017 Epigenetics plays a pivotal role in regulating gene expression in development, in response to cellular stress or in disease states, in virtually all cell types.
Adamo, Sergio +3 more
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ADP‐ribosyltransferase from beef liver which ADP‐ribosylates elongation factor‐2
FEBS Letters, 1984 Fragment A of diphtheria toxin and Pseudomonas toxin A intoxicate cells by ADP‐ribosylating the diphthamide residue of elongation factor‐2 (EF‐2) resulting in an inhibition of protein synthesis [1–3]. A cellular enzyme from polyoma virus transformed baby hamster kidney (pyBHK) cells ADP‐ribosylates EF‐2 in an identical manner [4].
Iglewski, Wallace J. +2 more
openaire +2 more sources
Selection of new appropriate reference genes for RT-qPCR analysis via transcriptome sequencing of cynomolgus monkeys (Macaca fascicularis). [PDF]
PLoS ONE, 2013 In the investigation of the expression levels of target genes, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most accurate and widely used method.
Sang-Je Park +10 more
doaj +1 more source