Results 111 to 120 of about 3,245 (169)
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Regeneration of the photoprotein aequorin
Nature, 1975THE photoprotein aequorin (molecular weight about 30,000) isolated from the bioluminescent jellyfish Aequorea aequorea1–3 emits blue light (λm a x 470 nm)4 by an intramolecular reaction in aqueous solution when Ca2+ is added, in the presence or absence of molecular oxygen1. This reaction has been postulated5 to follow the scheme in (Fig.
O, Shimomura, F H, Johnson
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The effect of alcohols on aequorin luminescence
Biochimica et Biophysica Acta (BBA) - General Subjects, 1986The effect of alcohols from ethanol to octanol on aequorin luminescence was biphasic; enhancement of both peak light and total photon yield at low concentrations, and inhibition of these parameters at high concentrations. The potency of alcohols to exert these effects was in the same order as the oil/water partition coefficients of the alcohols.
I R, Neering, M W, Fryer
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The Biological Bulletin, 1995
One day in the fall of 1960, shortly after my arrival at Princeton from Japan, Dr. Frank Johnson showed me a small jar containing a spoonful of white powder. He explained that the powder was a freeze dried “squeezate” made from the luminous jellyfish Aequorea, and that it would emit light when mixed with water.
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One day in the fall of 1960, shortly after my arrival at Princeton from Japan, Dr. Frank Johnson showed me a small jar containing a spoonful of white powder. He explained that the powder was a freeze dried “squeezate” made from the luminous jellyfish Aequorea, and that it would emit light when mixed with water.
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Microinjection of calcium into droplets of aequorin
Proceedings of the Royal Society of London. Series B. Biological Sciences, 1975Abstract Calcium ions were microinjected, ionophoretically or by pressure, into droplets of the photoprotein aequorin. For pulses less than about 1 s in duration, the amplitude and rate of rise of the light emitted as a result of the combination of calcium with aequorin, were directly proportional to the ionophoretic current flowing ...
K, Kusano, R, Miledi, J, Stinnakre
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Analytical Biochemistry, 1997
Biotinylated recombinant aequorin was used in the development of a heterogeneous bioluminescence binding assay for biotin. This assay is based on a competition between a biotinylated aequorin conjugate and biotin for the binding sites of avidin immobilized on solid particles.
A, Feltus, S, Ramanathan, S, Daunert
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Biotinylated recombinant aequorin was used in the development of a heterogeneous bioluminescence binding assay for biotin. This assay is based on a competition between a biotinylated aequorin conjugate and biotin for the binding sites of avidin immobilized on solid particles.
A, Feltus, S, Ramanathan, S, Daunert
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Aequorin: Its ionic specificity
Biochemical and Biophysical Research Communications, 1972Abstract The specificity of the cation-aequorin luminescent reaction was investigated. Contrary to present belief, this reaction was not specific for Ca 2+ and Sr 2+ . Over a dozen cations were capable of eliciting light emission from aequorin. Although these cations are not normally present in significant amounts in biological fluids, the present ...
K T, Izutsu +4 more
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Microdetermination of Calcium by Aequorin Luminescence
Science, 1963A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca ++ or Sr ++ , thus providing the basis for a simple, quantitative micromethod for the ...
O, Shimomura, F H, Johnson, Y, Saiga
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Thrombosis and Haemostasis, 1992
SummaryThe study compares the decay of intracellular luminescence activity (L max), the levels of basal [Ca2+]i in resting platelets, and agonist-induced peak [Ca2+]i-signals in platelets loaded with aequorin using the EGTA-, DMSO- and hypoosmotic shock treatment (HOST)-techniques.
R, Malmgren, S, Grunfelt, R, Joseph
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SummaryThe study compares the decay of intracellular luminescence activity (L max), the levels of basal [Ca2+]i in resting platelets, and agonist-induced peak [Ca2+]i-signals in platelets loaded with aequorin using the EGTA-, DMSO- and hypoosmotic shock treatment (HOST)-techniques.
R, Malmgren, S, Grunfelt, R, Joseph
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2006
Reporter proteins allow one to monitor cellular parameters that are involved in signal transduction, development, metabolic processes, and transport. There are targeting strategies available to direct the indicator protein exactly to the locale inside the organism from which information is desired.
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Reporter proteins allow one to monitor cellular parameters that are involved in signal transduction, development, metabolic processes, and transport. There are targeting strategies available to direct the indicator protein exactly to the locale inside the organism from which information is desired.
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Observation of Intracellular Ca2+ with Aequorin Luminescence
1991Publisher Summary This chapter focuses on the studies on Ca 2+ observation carried out by the aequorin– (video-intensified microscopes) VIM method. It also illustrates the principle of VIM and describes its modified version. The principal part of VIM is the VIM camera consisting of a two-dimensional photon-counting tube and an ordinary vidicon video
Y, Yoshimoto, Y, Hiramoto
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