Results 121 to 130 of about 2,993 (171)
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Microdetermination of Calcium by Aequorin Luminescence
Science, 1963A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca ++ or Sr ++ , thus providing the basis for a simple, quantitative micromethod for the ...
O, Shimomura, F H, Johnson, Y, Saiga
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The Biological Bulletin, 1995
One day in the fall of 1960, shortly after my arrival at Princeton from Japan, Dr. Frank Johnson showed me a small jar containing a spoonful of white powder. He explained that the powder was a freeze dried “squeezate” made from the luminous jellyfish Aequorea, and that it would emit light when mixed with water.
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One day in the fall of 1960, shortly after my arrival at Princeton from Japan, Dr. Frank Johnson showed me a small jar containing a spoonful of white powder. He explained that the powder was a freeze dried “squeezate” made from the luminous jellyfish Aequorea, and that it would emit light when mixed with water.
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Thrombosis and Haemostasis, 1992
SummaryThe study compares the decay of intracellular luminescence activity (L max), the levels of basal [Ca2+]i in resting platelets, and agonist-induced peak [Ca2+]i-signals in platelets loaded with aequorin using the EGTA-, DMSO- and hypoosmotic shock treatment (HOST)-techniques.
R, Malmgren, S, Grunfelt, R, Joseph
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SummaryThe study compares the decay of intracellular luminescence activity (L max), the levels of basal [Ca2+]i in resting platelets, and agonist-induced peak [Ca2+]i-signals in platelets loaded with aequorin using the EGTA-, DMSO- and hypoosmotic shock treatment (HOST)-techniques.
R, Malmgren, S, Grunfelt, R, Joseph
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2006
Reporter proteins allow one to monitor cellular parameters that are involved in signal transduction, development, metabolic processes, and transport. There are targeting strategies available to direct the indicator protein exactly to the locale inside the organism from which information is desired.
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Reporter proteins allow one to monitor cellular parameters that are involved in signal transduction, development, metabolic processes, and transport. There are targeting strategies available to direct the indicator protein exactly to the locale inside the organism from which information is desired.
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Observation of Intracellular Ca2+ with Aequorin Luminescence
1991Publisher Summary This chapter focuses on the studies on Ca 2+ observation carried out by the aequorin– (video-intensified microscopes) VIM method. It also illustrates the principle of VIM and describes its modified version. The principal part of VIM is the VIM camera consisting of a two-dimensional photon-counting tube and an ordinary vidicon video
Y, Yoshimoto, Y, Hiramoto
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The Journal of Biochemistry, 2018
cf3-Aequorin is one of the semi-synthetic aequorins that was produced by replacing 2-peroxycoelenterazine (CTZ-OOH) in native aequorin with a 2-peroxycoelenterazine analog, and it was prepared using the C2-modified trifluoromethyl analog of coelenterazine (cf3-CTZ) and the histidine-tagged apoaequorin expressed in Escherichia coli cells.
Satoshi Inouye +4 more
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cf3-Aequorin is one of the semi-synthetic aequorins that was produced by replacing 2-peroxycoelenterazine (CTZ-OOH) in native aequorin with a 2-peroxycoelenterazine analog, and it was prepared using the C2-modified trifluoromethyl analog of coelenterazine (cf3-CTZ) and the histidine-tagged apoaequorin expressed in Escherichia coli cells.
Satoshi Inouye +4 more
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Spectral components of bioluminescence of aequorin and obelin
Journal of Photochemistry and Photobiology B: Biology, 2008Complex bioluminescence spectra of photoproteins from marine coelenterates - jellyfish Aequorea victoria and hydroid Obelia longissima, and photoluminescence spectra of the bioluminescent reaction products (Ca(2+)-discharged photoproteins) were deconvolved into components.
Nadezhda V, Belogurova +3 more
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The discovery of aequorin and green fluorescent protein
Journal of Microscopy, 2005We discovered aequorin and green fluorescent protein (GFP) in 1961 from the same species of jellyfish (Shimomura et al ., 1962). Our target was a luminescent substance, aequorin, and GFP was isolated as a by-product of aequorin owing to its bright conspicuous fluorescence.
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Targeting of aequorin for calcium monitoring in intracellular compartments
Journal of Bioluminescence and Chemiluminescence, 1994AbstractWe have recently developed a new method for monitoring Ca2+ concentrations in defined cell compartments. The cDNA encoding the Ca2+‐sensitive photoprotein aequorin has been modified in order to include specific targeting sequences and expressed in eukaryotic cells; the recombinant protein, specifically located inside the cells, has allowed the ...
BRINI, MARISA +5 more
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Molecular Reproduction and Development, 2014
SUMMARYIn this review, we take a retrospective look at the discovery and utilization of the Ca2+‐sensitive bioluminescent protein complex, aequorin. We do consider the contribution it has made to our understanding of the natural phenomenon of bioluminescence, but it is in the application of extracted and purified aequorin as a reporter of Ca2+dynamics ...
Webb, Sarah E. +2 more
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SUMMARYIn this review, we take a retrospective look at the discovery and utilization of the Ca2+‐sensitive bioluminescent protein complex, aequorin. We do consider the contribution it has made to our understanding of the natural phenomenon of bioluminescence, but it is in the application of extracted and purified aequorin as a reporter of Ca2+dynamics ...
Webb, Sarah E. +2 more
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