Results 121 to 130 of about 3,245 (169)
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The Journal of Biochemistry, 2018
cf3-Aequorin is one of the semi-synthetic aequorins that was produced by replacing 2-peroxycoelenterazine (CTZ-OOH) in native aequorin with a 2-peroxycoelenterazine analog, and it was prepared using the C2-modified trifluoromethyl analog of coelenterazine (cf3-CTZ) and the histidine-tagged apoaequorin expressed in Escherichia coli cells.
Satoshi Inouye +4 more
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cf3-Aequorin is one of the semi-synthetic aequorins that was produced by replacing 2-peroxycoelenterazine (CTZ-OOH) in native aequorin with a 2-peroxycoelenterazine analog, and it was prepared using the C2-modified trifluoromethyl analog of coelenterazine (cf3-CTZ) and the histidine-tagged apoaequorin expressed in Escherichia coli cells.
Satoshi Inouye +4 more
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Targeting of aequorin for calcium monitoring in intracellular compartments
Journal of Bioluminescence and Chemiluminescence, 1994AbstractWe have recently developed a new method for monitoring Ca2+ concentrations in defined cell compartments. The cDNA encoding the Ca2+‐sensitive photoprotein aequorin has been modified in order to include specific targeting sequences and expressed in eukaryotic cells; the recombinant protein, specifically located inside the cells, has allowed the ...
BRINI, MARISA +5 more
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Spectral components of bioluminescence of aequorin and obelin
Journal of Photochemistry and Photobiology B: Biology, 2008Complex bioluminescence spectra of photoproteins from marine coelenterates - jellyfish Aequorea victoria and hydroid Obelia longissima, and photoluminescence spectra of the bioluminescent reaction products (Ca(2+)-discharged photoproteins) were deconvolved into components.
Nadezhda V, Belogurova +3 more
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Targeting, Expressing and Calibrating Recombinant Aequorin
1999The wide diffusion of molecular biology techniques, with the possibility of modifying and expressing in virtually all cell types exogenous cDNAs, has been responsible for the large expansion in the use of protein probes in cell biology. Two groups of reporter proteins are currently employed, derived from the wide variety of bioluminescent organisms ...
BRINI, MARISA, Pinton P, Bastianutto C.
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Molecular Reproduction and Development, 2014
SUMMARYIn this review, we take a retrospective look at the discovery and utilization of the Ca2+‐sensitive bioluminescent protein complex, aequorin. We do consider the contribution it has made to our understanding of the natural phenomenon of bioluminescence, but it is in the application of extracted and purified aequorin as a reporter of Ca2+dynamics ...
Webb, Sarah E. +2 more
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SUMMARYIn this review, we take a retrospective look at the discovery and utilization of the Ca2+‐sensitive bioluminescent protein complex, aequorin. We do consider the contribution it has made to our understanding of the natural phenomenon of bioluminescence, but it is in the application of extracted and purified aequorin as a reporter of Ca2+dynamics ...
Webb, Sarah E. +2 more
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The discovery of aequorin and green fluorescent protein
Journal of Microscopy, 2005We discovered aequorin and green fluorescent protein (GFP) in 1961 from the same species of jellyfish (Shimomura et al ., 1962). Our target was a luminescent substance, aequorin, and GFP was isolated as a by-product of aequorin owing to its bright conspicuous fluorescence.
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2012
There are special proteins that do produce light by themselves (bioluminescence; no excitation light is necessary). An example is the aequorin-GFP-complex (GFP, green fluorescent protein) that naturally occurs in the jellyfish Aequorea victoria. Interestingly, the famous paper by Shimomura that identified GFP as a protein only mentioned GFP in one ...
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There are special proteins that do produce light by themselves (bioluminescence; no excitation light is necessary). An example is the aequorin-GFP-complex (GFP, green fluorescent protein) that naturally occurs in the jellyfish Aequorea victoria. Interestingly, the famous paper by Shimomura that identified GFP as a protein only mentioned GFP in one ...
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Properties of the bioluminescent protein aequorin
Biochemistry, 1969O, Shimomura, F H, Johnson
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Molecular weight of the photoprotein aequorin
Biochemistry, 1971Y, Kohama, O, Shimomura, F H, Johnson
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