Results 71 to 80 of about 2,332 (298)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Experimental and Computational Evaluation of Five Hole Five Probe Flow Analyzer for Subsonic Wind Calibration

International Journal of Aviation, Aeronautics, and Aerospace, 2020
It is very essential to predict the flow and its related parameters to understand real life fluid flow. The real-life fluid problems are studied with wind tunnels which gives very efficient results in practice. But in the case of wind tunnel studies flow
Akhila Rupesh, J V Muruga Lal Jeyan
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Digital particle image velocimetry measurements of the downwash distribution of a desert locust Schistocerca gregaria [PDF]

, 2006
Actuator disc models of insect flight are concerned solely with the rate of momentum transfer to the air that passes through the disc. These simple models assume that an even pressure is applied across the disc, resulting in a uniform downwash ...
Taylor, Graham K., Lawson, NJ, Taylor, GK, Taylor, Graham, Bomphrey, Richard J., Thomas, Adrian L. R., Bomphrey, RJ, Lawson, Nicholas J., Thomas, AL   +8 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Wind Tunnel Investigation of Transient Propeller Loads for Non-Axial Inflow Conditions

Aerospace
Recent developments in electrical Vertical Take-off and Landing (eVTOL) vehicles show the need for a better understanding of transient aero-mechanical propeller loads for non-axial inflow conditions.
Catharina Moreira, Nikolai Herzog, Christian Breitsamter   +2 more
doaj   +1 more source

Impact of a Reverse Delta Type Add-on Device on the Flap-tip Vortex of a Wing

International Journal of Aviation, Aeronautics, and Aerospace, 2016
The effect of interactions of vortices produced by an outboard flap-tip of a half-span wing (NACA 23012 in landing configuration) and a slender reverse delta type add-on device, placed in the proximity of the outboard flap-tip, on the upper surface of ...
Afaq Altaf, Tan Boon Thong, Ashraf Ali Omar, Waqar Asrar   +3 more
doaj   +1 more source

The Detrimental Effect of Rainfall on Performance and Stability Characteristics of Aircrafts-A Comprehensive Review

International Journal of Aviation, Aeronautics, and Aerospace, 2020
The rapidly growing global market demands faster transit of human beings and goods even in unfavorable weather conditions of rainfall. The paper gives an overview of rain research methodologies, mathematical models to track raindrops in the flow field ...
Praneeth HR, Amit Kumar Thakur
doaj   +1 more source