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Optimization of a plasmid electroporation protocol for Aeromonas salmonicida subsp. salmonicida

Journal of Microbiological Methods, 2014
Aeromonas salmonicida subsp. salmonicida is a major fish pathogen. Molecular tools are required to study the virulence and genomic stability of this bacterium. An efficient electroporation-mediated transformation protocol for A. salmonicida subsp. salmonicida would make genetic studies faster and easier.
Stéphanie, Dallaire-Dufresne   +6 more
openaire   +2 more sources

RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila

Journal of Applied Bacteriology, 1995
The randomly amplified polymorphic DNA (RAPD) technique was used to analyse the genetic differentiation of 13 strains of Aeromonas salmonicida subsp. salmonicida , and seven strains of Aer.
M, Miyata   +4 more
openaire   +2 more sources

The molecular biology of Aeromonas salmonicida

Annual Review of Fish Diseases, 1995
Abstract The fish pathogen, Aeromonas salmonicida, has been the focus of a number of molecular genetic studies designed to characterize the microorganism and its pathogenesis. The paracrystalline surface protein layer (A-layer) of A. salmonicida has been studied in considerable detail. The A-layer gene, vapA, has been cloned and sequenced and studies
Brian Noonan, Trevor J. Trust
openaire   +1 more source

Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide

Carbohydrate Research, 2006
The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [carbohydrate: see text] where R=alpha-D-Galp-(1-->4)-beta-D-GalpNAc-(1--> or alpha-D ...
Zhan, Wang   +3 more
openaire   +2 more sources

Structural characterization of the lipid A region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide

Carbohydrate Research, 2006
The lipid A components of Aeromonas salmonicida subsp. salmonicida from strains A449, 80204-1 and an in vivo rough isolate were isolated by mild acid hydrolysis of the lipopolysaccharide. Structural studies carried out by a combination of fatty acid, electrospray ionization-mass spectrometry and nuclear magnetic resonance analyses confirmed that the ...
Zhan, Wang   +2 more
openaire   +2 more sources

Direct analysis of starved Aeromonas salmonicida

Journal of Fish Diseases, 1996
AbstractSurvival of Aeromonas salmonicida was monitored during prolonged incubation in either distilled water or lake water. Culturability was determined from colony forming units enumerated on tryptone soy agar, whilst flow cytometry was used for direct analysis of viable cells after staining with fluorescent dyes which differentially stained bacteria
Deere, D.   +3 more
openaire   +1 more source

A lipopolysaccharide-specific bacteriophage for Aeromonas salmonicida

Canadian Journal of Microbiology, 1983
Cell wall lipopolysaccharide (LPS) was identified as the receptor for the Aeromonas salmonicida bacteriophage strain 55R-1. Mutants of A. salmonicida resistant to phage 55R-1 were unable to adsorb phage 55R-1 and were shown to be defective in LPS structure. Purified A.
E E, Ishiguro   +4 more
openaire   +2 more sources

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