Results 191 to 200 of about 1,855,891 (316)

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification. [PDF]

Int J Pept Res Ther, 2021
Barredo-Vacchelli GR, Giudicessi SL, Martínez-Ceron MC, Cascone O, Camperi SA.   +4 more
europepmc   +1 more source

Transport property predictions for laser resonance chromatography on Rf$^+$ (Z = 104) [PDF]

arXiv
We propose a theoretically designed laser resonance chromatography (LRC) experiment on Rf$^+$ (Z = 104) drifting in He buffer gas. To this end, we first developed a four-level rate equation model that simulates the optical pumping of Rf$^+$ from its ground state, $^2$D$_{3/2}$ (7s$^2$6d$^1$), to the metastable $^4$F$_{3/2}$ (7s$^1$6d$^2$) state via ...
arxiv  

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

MethodsX
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza, Roberto Verardo, Claudio Schneider   +2 more
doaj  

Protein purification with light via a genetically encoded azobenzene side chain

Nature Communications
Affinity chromatography is the method of choice for the rapid purification of proteins from cell extracts or culture supernatants. Here, we present the light-responsive Azo-tag, a short peptide comprising p-(phenylazo)-L-phenylalanine (Pap), whose side ...
Peter Mayrhofer, Markus R. Anneser, Kristina Schira, Carina A. Sommer, Ina Theobald, Martin Schlapschy, Stefan Achatz, Arne Skerra   +7 more
doaj   +1 more source

Enriching Cysteine-Containing Peptides Using a Sulfhydryl-Reactive Alkylating Reagent with a Phosphonic Acid Group and Immobilized Metal Affinity Chromatography. [PDF]

J Proteome Res, 2023
Liu X, Rossio V, Gygi SP, Paulo JA.
europepmc   +1 more source

Affinity chromatography of rabbit liver .BETA.-glucuronidase.

, 1979
Nobuko Iino, Kazuo Yoshida
openalex   +2 more sources

Molecular engineering of cyclic azobenzene-peptide hybrid ligands for the purification of human blood Factor VIII via photo-affinity chromatography. [PDF]

Adv Funct Mater, 2023
Prodromou R, Moore B, Chu W, Deal H, Miguel AS, Brown AC, Daniele MA, Pozdin V, Menegatti S.   +8 more
europepmc   +1 more source

Purification of biologically active globin mRNA using cDNA-cellulose affinity chromatography.

, 1977
T.Gordon Wood, Jerry B. Lingrel
openalex   +1 more source

Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen. [PDF]

Int J Mol Sci, 2022
Popovic G, Kirby NC, Dement TC, Peterson KM, Daub CE, Belcher HA, Guthold M, Offenbacher AR, Hudson NE.   +8 more
europepmc   +1 more source

Identification of Clusterin as a Major ABri- and ADan-Binding Protein Using Affinity Chromatography. [PDF]

Methods Mol Biol, 2022
Rostagno A, Calero M, Ghiso J.
europepmc   +1 more source