Results 191 to 200 of about 26,612 (231)
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AFLP Linkage Map of the OomycetePhytophthora infestans
Fungal Genetics and Biology, 1997Here we present the first comprehensive genetic linkage map of the heterothallic oomycetous plant pathogen Phytophthora infestans. The map is based on polymorphic DNA markers generated by the DNA fingerprinting technique AFLP (Vos et al., 1995, Nucleic Acids Res. 23: 4407-4414). AFLP fingerprints were made from single zoospore progeny and 73 F1 progeny
Francine Govers +2 more
exaly +7 more sources
Theoretical and Applied Genetics, 1999
AFLP markers were evaluated for their usefulness in the genetic analysis of sugarbeet and wild Beta species. Accessions of ten different sugarbeet breeding lines and five wild beets were screened using 256 primer combinations. Of the 11 309 bands investigated, 96.4% were polymorphic among the accessions.
M. Hansen +3 more
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AFLP markers were evaluated for their usefulness in the genetic analysis of sugarbeet and wild Beta species. Accessions of ten different sugarbeet breeding lines and five wild beets were screened using 256 primer combinations. Of the 11 309 bands investigated, 96.4% were polymorphic among the accessions.
M. Hansen +3 more
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Tissue Antigens, 1991
Abstract: We have described a practical and inexpensive method whereby any individual can be typed and assigned to any of the 14 generic HLA‐DR types: DR1, DRwl5, DRwl6, DRwl7, DRwl8, DR4, DRwll, DRwl2, DRwl3, DRwl4, DR7, DRw8, DR9 and DRw10. Previous methods to type these specificities include ‐ among others ‐ serology, conventional RFLP, PCR ...
I, Yunis, M, Salazar, E J, Yunis
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Abstract: We have described a practical and inexpensive method whereby any individual can be typed and assigned to any of the 14 generic HLA‐DR types: DR1, DRwl5, DRwl6, DRwl7, DRwl8, DR4, DRwll, DRwl2, DRwl3, DRwl4, DR7, DRw8, DR9 and DRw10. Previous methods to type these specificities include ‐ among others ‐ serology, conventional RFLP, PCR ...
I, Yunis, M, Salazar, E J, Yunis
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AFLP variation in 25 Avena species
Theoretical and Applied Genetics, 2008Current molecular characterization of ex situ plant germplasm has placed more emphasis on cultivated gene pools and less on exotic gene pools representing wild relative species. This study attempted to characterize a selected set of germplasm accessions representing various Avena species with the hope to establish a reference set of exotic oat ...
Yong-Bi, Fu, David J, Williams
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AFLP fingerprinting for paternity testing in ducks
British Poultry Science, 20071. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2.
Huang, C.W. +5 more
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AFLP data and the origins of domesticated crops
Genome, 2003Amplified fragment length polymorphism (AFLP) datasets have been used to construct neighbor-joining trees from which monophyletic origins for crops such as einkorn wheat, barley, and emmer wheat have been inferred. We simulated several different multiple domestication scenarios for an imaginary cereal crop and examined the resulting domesticated ...
Allaby, Robin G., Brown, Terence A.
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The status of AFLP in the genomics era and a pipeline for converting AFLPs into single-locus markers
Molecular Breeding, 2014Even though next-generation sequencing (NGS) has now become the predominant state-of-the-art technique for genotyping populations, amplified fragment length polymorphism (AFLP) DNA fingerprinting is still a relevant method, thanks to its versatility, cost-effectiveness, independence of prior sequence information and broad applicability. Even though the
Zhengfeng Zhang +2 more
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AbstractThis unit presents an alternative to differential display that allows the quantification of transcripts, based on AFLP‐fingerprinting of double‐stranded cDNA. The protocol described includes the following steps: the isolation of poly(A)+ RNA from total RNA, the synthesis of double‐stranded cDNA, the preparation of template fragments by ...
Pieter, Vos, Patrick, Stanssens
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AFLP™ markers for DNA fingerprinting in cattle
Animal Genetics, 1997This work reports on use of the recently described amplified fragment length polymorphism (AFLP) technology for DNA fingerprinting in cattle. The AFLP technology produces molecular markers through the high‐stringency polymerase chain reaction (PCR)‐amplification of restriction fragments that are ligated to synthetic adapters and amplified using primers,
AJMONE MARSAN P +5 more
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Multilocus Profiling with AFLP, ISSR, and SAMPL
2013Molecular markers which sample multiple loci simultaneously, like amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSR), and selective amplification of microsatellite polymorphic loci (SAMPL), produce highly informative fingerprints due to their high effective multiplex ratio and expected heterozygosity.
Luis F, Goulao, Cristina M, Oliveira
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