Results 21 to 30 of about 237,834 (284)

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

Stem Cell Research & Therapy, 2023
Background CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such “disease in a dish” models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access ...
Elie Frank, Michel Cailleret, Constantin Nelep, Pascal Fragner, Jérome Polentes, Elise Herardot, Lina El Kassar, Karine Giraud-Triboult, Christelle Monville, Karim Ben M’Barek   +9 more
doaj   +1 more source

Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset

Journal of Cachexia, Sarcopenia and Muscle, 2021
Background Duchenne muscular dystrophy (DMD) causes severe disability of children and death of young men, with an incidence of approximately 1/5000 male births.
Virginie Mournetas, Emmanuelle Massouridès, Jean‐Baptiste Dupont, Etienne Kornobis, Hélène Polvèche, Margot Jarrige, Alan R.L. Dorval, Maxime R.F. Gosselin, Antigoni Manousopoulou, Spiros D. Garbis, Dariusz C. Górecki, Christian Pinset   +11 more
doaj   +1 more source

SISTEMA: A large and standardized collection of transcriptome data sets for human pluripotent stem cell research

iScience, 2021
Summary: Human pluripotent stem cells have ushered in an exciting new era for disease modeling, drug discovery, and cell therapy development. Continued progress toward realizing the potential of human pluripotent stem cells will be facilitated by robust ...
Margot Jarrige, Hélène Polvèche, Alexandre Carteron, Stéphane Janczarski, Marc Peschanski, Didier Auboeuf, Cécile Martinat   +6 more
doaj   +1 more source

Non-contact atomic force microscope: Modeling and simulation using van der Pol averaging method [PDF]

Известия высших учебных заведений: Прикладная нелинейная динамика, 2021
Topic and aim. One of the tools which are extremely useful and valuable for creating a topography of surfaces, measuring forces, and manipulating material with nano-meter-scale features is the Atomic force microscope (AFM).
Bahrami, Mohammad Reza
doaj   +1 more source

Synthesis of Copper(II) Schiff Base Complex and Its Mixed Thin Layer with ZnO Nanoparticles [PDF]

Iranian Journal of Chemistry & Chemical Engineering, 2021
Cu(II) complex derived from Synthesis of N-((1H-pyrrol-2-yl)methylene)-4-methoxyaniline Schiff base(PMMA) was studied by UV-Vis, IR spectra, and EDAX.
Charles A, Sivaraj K, Krishnaraj S
doaj   +1 more source

Pluripotent Stem Cell-Based Drug Screening Reveals Cardiac Glycosides as Modulators of Myotonic Dystrophy Type 1

iScience, 2019
Summary: There is currently no treatment for myotonic dystrophy type 1 (DM1), the most frequent myopathy of genetic origin. This progressive neuromuscular disease is caused by nuclear-retained RNAs containing expanded CUG repeats.
Yves Maury, Pauline Poydenot, Benjamin Brinon, Lea Lesueur, Jacqueline Gide, Sylvain Roquevière, Julien Côme, Hélène Polvèche, Didier Auboeuf, Jérome Alexandre Denis, Geneviève Pietu, Denis Furling, Marc Lechuga, Sandrine Baghdoyan, Marc Peschanski, Cécile Martinat   +15 more
doaj   +1 more source

Pluripotent Stem Cells in Disease Modeling and Drug Discovery for Myotonic Dystrophy Type 1

Cells, 2023
Myotonic dystrophy type 1 (DM1) is a progressive multisystemic disease caused by the expansion of a CTG repeat tract within the 3′ untranslated region (3′ UTR) of the dystrophia myotonica protein kinase gene (DMPK).
Noémie Bérenger-Currias, Cécile Martinat, Sandrine Baghdoyan   +2 more
doaj   +1 more source

Applications of AFM in pharmaceutical sciences [PDF]

, 2015
Atomic force microscopy (AFM) is a high-resolution imaging technique that uses a small probe (tip and cantilever) to provide topographical information on surfaces in air or in liquid media.
James R. Smith, Smith, James R., Dimitrios A. Lamprou, Lamprou, Dimitrios   +3 more
core   +1 more source

Experimental investigation on the effet of input paramètres on surface roughness and MRR of abrasive flow machining process [PDF]

E3S Web of Conferences, 2023
A crucial and costly step in the whole manufacturing process is the precision and super finishing procedure. A step of final finishing is involved in the production of precision components.
Rao P. Kondala, Janardhana G. Ranga
doaj   +1 more source

Probing the determinants of cellular elasticity by AFM [PDF]

, 2012
Cells within tissues continuously encounter mechanical challenges to which they respond by remodelling their cytoskeleton. Cellular interactions as well as the microenvironment regulate the cell’s mechanical response under physiological and pathological ...
Plodinec, Marija
core   +1 more source