Results 1 to 10 of about 5,412,420 (313)

Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinoceros

iScience, 2022
Summary: Less than 80 Sumatran rhinos (SR, Dicerorhinus sumatrensis) are left on earth. Habitat loss and limited breeding possibilities are the greatest threats to the species and lead to a continuous population decline.
Vera Zywitza, Silke Frahm, Norman Krüger, Anja Weise, Frank Göritz, Robert Hermes, Susanne Holtze, Silvia Colleoni, Cesare Galli, Micha Drukker, Thomas B. Hildebrandt, Sebastian Diecke   +11 more
doaj   +1 more source

Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction

Scientific Reports, 2022
The northern white rhinoceros (NWR) is probably the earth’s most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro.
Vera Zywitza, Ejona Rusha, Dmitry Shaposhnikov, Jorge Ruiz-Orera, Narasimha Telugu, Valentyna Rishko, Masafumi Hayashi, Geert Michel, Lars Wittler, Jan Stejskal, Susanne Holtze, Frank Göritz, Robert Hermes, Jichang Wang, Zsuzsanna Izsvák, Silvia Colleoni, Giovanna Lazzari, Cesare Galli, Thomas B. Hildebrandt, Katsuhiko Hayashi, Sebastian Diecke, Micha Drukker   +21 more
doaj   +1 more source

Generation of a human embryonic stem cell line (WAe009-A-78) carrying homozygous TBX18 knockout

Stem Cell Research, 2022
T-Box Transcription Factor 18 is a member of the T-box family, encoding TBX18 protein. As a transcriptional repressor, it related to developmental processes of a majority of tissues and organs and plays crucial part in the embryonic development of ...
Qi Xu, Tiantian Zhao, Yongshuai Zhang, Wei Fan, Yuting Yan, Feng Lan   +5 more
doaj   +1 more source

ASSURED-optimized CRISPR protocol for knockout/SNP knockin in hiPSCs

STAR Protocols, 2023
Summary: CRISPR-Cas9 technology coupled with human induced pluripotent stem cells allows precise disease modeling in pluripotent cells and subsequently derived specialized cell types.
Katarzyna A. Ludwik, Narasimha Telugu, Sandra Schommer, Harald Stachelscheid, Sebastian Diecke   +4 more
doaj   +1 more source

Generation of a human embryonic stem cell line WAe009-A-79 carrying a long QT syndrome mutation in KCNQ1

Stem Cell Research, 2023
The voltage-gated potassium channel KvLQT1 encoded by KCNQ1 plays an important role in the repolarization of myocardial action potentials. KCNQ1 mutations can cause Long QT syndrome type 1 (LQT1), which is considered to be the most common causative gene ...
Hongyue Wang, Tianwei Guo, Feng Lan
doaj   +1 more source

Porcine OCT4 reporter system as a tool for monitoring pluripotency states

Journal of Animal Reproduction and Biotechnology, 2021
Pluripotent stem cells could self-renew and differentiate into various cells. In particular, porcine pluripotent stem cells are useful for preclinical therapy, transgenic animals, and agricultural usage. These stem cells have naïve and primed pluripotent
Seung-Hun Kim, Chang-Kyu Lee
doaj   +1 more source

Non-coding microRNAs for cardiac regeneration: Exploring novel alternatives to induce heart healing

Non-coding RNA Research, 2017
In recent years, different studies have revealed that adult mammalian cardiomyocytes have the capacity to self-renew under homeostatic conditions and after myocardial injury.
Elena Garreta, Patricia Prado, Juan Carlos Izpisua Belmonte, Nuria Montserrat   +3 more
doaj   +1 more source

Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells

Stem Cells International, 2016
Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development.
Gergo Kovacs, Viktoria Szabo, Melinda K. Pirity   +2 more
doaj   +1 more source

Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality

Frontiers in Cell and Developmental Biology, 2022
The technique of induced pluripotent stem cells has significant application value in breeding and preserving the genetic integrity of fish species. However, it is still unclear whether the chemically induced pluripotent stem cells can be induced from non-
Wenting Xu, Wenting Xu, Huajin Li, Huajin Li, Liangyue Peng, Liangyue Peng, Liyu Pu, Liyu Pu, Sijia Xiang, Sijia Xiang, Yue Li, Yue Li, Leiting Tao, Leiting Tao, Wenbin Liu, Wenbin Liu, Jinhui Liu, Jinhui Liu, Yamei Xiao, Yamei Xiao, Shaojun Liu, Shaojun Liu   +21 more
doaj   +1 more source

Non-human primate pluripotent stem cells for the preclinical testing of regenerative therapies

Neural Regeneration Research, 2022
Non-human primates play a key role in the preclinical validation of pluripotent stem cell-based cell replacement therapies. Pluripotent stem cells used as advanced therapy medical products boost the possibility to regenerate tissues and organs affected ...
Ignacio Rodriguez-Polo, Rüdiger Behr
doaj   +1 more source