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Ring chromosome 15: phenotype, Ag-NOR analysis, secondary aneuploidy, and associated chromosome instability

Cytogenetic and Genome Research, 1980
Cytogenetic analysis of a 15 month old girl evaluated for severe developmental delay and acral skeletal hypoplasia revealed a predominant 46, XX, r(15) karyotype. Prophase banding analysis showed minimal deletion of the ring chromosome (breakpoints pl2 and q26), while silver staining showed it to have an active nucleolus organizing region, multiple ...
D H, Ledbetter   +4 more
openaire   +3 more sources

A new version of the Ag−NOR technique. A combination with DAPI staining

The Histochemical Journal, 1996
The Ag-NOR staining technique is widely used for visualizing nucleolar organizer regions (NORs) in various plant and animal tissues. We describe a simple and time-saving combination of Ag-NOR staining with DNA detection by fluorescence microscopy. This modification was tested on cultured cells and semi-thin sections of plastic-embedded tissues.
S M, Biliński, B, Bilińska
openaire   +3 more sources

Visualization of Ag-NOR proteins on nucleolar transcriptional units in molecular spreads

Chromosoma, 1982
Application of NOR silver staining to Pleurodeles oocyte nuclei showed selective silver deposits on the fibrillar part of the nucleoli in situ. This staining was adapted to nucleoli spread on grids, by a procedure which allowed the spreading of transcription units from both nucleoli and lampbrush chromosomes on the same grids.
N, Angelier   +2 more
openaire   +3 more sources

Replication, C and Ag-NOR chromosome-banding in two anguilliform fish species

Marine Biology, 1995
The chromosomes of the Conger conger and Echelus myrus (Anguilliformes: Teleostei), were analysed by conventional (C and Ag-NOR) and by replication-banding techniques. Both species had a diploid number of 38 and one chromosome pair with C-positive nucleolar organizer regions (NOR).
A. Amores, J. Bejar, M. C. Alvarez
openaire   +2 more sources

Ag-NOR staining in mouse sarcoma ascites cells

Journal of Cancer Research and Clinical Oncology, 1983
In sarcoma cells, the nucleoli are small, ill-defined, and are present in large numbers making it difficult to stain them by classical staining procedures. A modified Ag-NOR technique has been applied to detect the nucleolus and the nucleolar-organizing region in the chromosome of a mouse sarcoma-180 ascites cell-line. The cells were found to have 8-14
A, Chaudhuri, S, Ghosh
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Nucleolar organizer regions (Ag-NORs) in multinuclear osteoclasts

Journal of Bone and Mineral Metabolism, 1998
Osteoclasts are multinuclear bone-resorbing cells. Little is known about regulation of gene expression in these cells and how it changes during the aging of an osteoclast, which is indicated by an increasing number of nuclei. Silver staining of nucleolar organizer regions, so-called Ag-NORs, allows us to assess the activity of rRNA expression.
Narelle Roennfeldt   +3 more
openaire   +1 more source

Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes

2023
Silver nitrate staining to evidence the location of nucleolar organizer regions (Ag-NORs) in chromosomes is widely used as a classical method in plant cytogenetics. Here, we present the most used procedures and highlight some aspects in terms of their replicability by plant cytogeneticists.
Claudio, Palma-Rojas   +2 more
openaire   +2 more sources

“Ag-NORs” are not always true NORs: new evidence in mammals

Cytogenetic and Genome Research, 2002
In spite of uncertainty about the biochemical processes involved, silver staining is a widely used technique for assessing the locations of active NORs in eukaryotic genomes in general, and in mammalian genomes in particular. However, following a previous study of hedgehog chromosomes, we present here a second example from two gerbil species (Rodentia,
G, Dobigny   +3 more
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Functional Sexual Differences in Rat Mammillary Bodies: A Quantitative Ag-NOR Study

Cells Tissues Organs, 1996
The mammillary bodies of the posterior hypothalamus are one of the CNS structures in which structural sexual dimorphism has already been described. This study quantifies the argyrophilic nucleolar organizer regions (NORs) of the neurons from this region in the male and female rats, the latter during two major phases of the estrous cycle (estrus ...
S, González-González   +3 more
openaire   +2 more sources

The nucleolar organizer regions associated protein (Ag-NORs) in salivary gland tumors

International Journal of Oral and Maxillofacial Surgery, 1989
A silver colloid technique used to identify nucleolar organizer regions associated protein (Ag-NORs) has been applied to 20 salivary gland tumors. The method was readily applicable to the preparations of paraffin-embedded sections and the Ag-NORs were enumerated with ease.
K, Matsumura   +3 more
openaire   +2 more sources

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