The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Genetics and Molecular Biology, 2014 Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49.
Tiago Marafiga Degrandi +9 more
doaj +1 more source
Molecular Oncology, EarlyView.Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla +9 more
wiley +1 more source
The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia [PDF]
, 2012 Objective : To determine the proliferative activity of cervical intraepithelial lesions to assist in the diagnosis and treatment of these lesions through the use of silver stained nucleolar organizer region (Ag +– NOR). Nucleolar organizer regions (NOR)
Faisal, Ghasak Ghazi +1 more
core
Ag-NOR" proteins are present when transcription is impaired
, 1984 The use of nucleolar silver staining in plant cells in conditions of impaired transcription, either natural (quiescent cells) or induced (actinomycin D, ethidium bromide and cordycepin treatments), shows that nucleoli with very low transcription rates ...
Fernández-Gómez, M. E. +3 more
core +1 more source
Ag-NOR staining and satellite association in bone marrow cells from patients with mycosis fungoides [PDF]
, 2021 Silver staining of nucleolus organizing regions (Ag-NORs) of acrocentric chromosomes and the frequency of satellite association (SA) in bone marrow (BM) cells from 7 patients with mycosis fungoides (MF), were studied.
Slavutsky, Irma +1 more
core
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
A new karyotypic formula for the genus Amphisbaena (Squamata: Amphisbaenidae) [PDF]
Phyllomedusa: Journal of Herpetology, 2010 To contribute to the knowledge of chromosome evolution in Amphisbaena, we describe the karyotype constitution and the NOR location of Amphisbaena bolivica Mertens, 1929.
Camila Falcione, Alejandra Hernando
doaj
Amount variability of total and individual Ag-NOR proteins in cells stimulated to proliferate.
, 1995 Ribosomal genes are associated with a subset of acidic proteins called Ag-NOR proteins. The amount of nucleolar Ag-NOR proteins varies, depending on nucleolar activity and/or cell proliferation.
M Derenzini +4 more
core +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source