Results 21 to 30 of about 19,284 (137)
Biochemical characterization of the interaction between KRAS and Argonaute 2
Biochemistry and Biophysics Reports, 2022 Oncogenic mutations in KRAS result in a constitutively active, GTP-bound form that in turn activates many proliferative pathways. However, because of its compact and simple architecture, directly targeting KRAS with small molecule drugs has been ...
Jessica J. Waninger +8 more
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NF90 interacts with components of RISC and modulates association of Ago2 with mRNA [PDF]
BMC Biology, 2021 Abstract Background Nuclear factor 90 (NF90) is a double-stranded RNA-binding protein involved in a multitude of different cellular mechanisms such as transcription, translation, viral infection, and mRNA stability. Recent data suggest that NF90 might influence the abundance of target mRNAs in the cytoplasm through miRNA-
Giuseppa Grasso +6 more
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Analysis of chimeric reads characterises the diverse targetome of AGO2-mediated regulation
Scientific Reports, 2023 Argonaute proteins are instrumental in regulating RNA stability and translation. AGO2, the major mammalian Argonaute protein, is known to primarily associate with microRNAs, a family of small RNA ‘guide’ sequences, and identifies its targets primarily ...
Vaclav Hejret +6 more
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Sumoylation of human argonaute 2 at lysine-402 regulates its stability. [PDF]
PLoS ONE, 2014 Gene silencing by small RNAs has emerged as a powerful post-transcriptional regulator of gene expression, however processes underlying regulation of the small RNA pathway in vivo are still largely elusive.
Umut Sahin +4 more
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Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. [PDF]
PLoS ONE, 2009 Fragile X Syndrome is caused by the silencing of the Fragile X Mental Retardation gene (FMR1). Regulating dosage of FMR1 levels is critical for proper development and function of the nervous system and germ line, but the pathways responsible for ...
Anita S-R Pepper +3 more
doaj +1 more source
Plants, 2021 RNA silencing is a sequence specific post-transcriptional mechanism regulating important biological processes including antiviral defense in plants. Argonaute (AGO) proteins, the catalytic subunits of the silencing complexes, are loaded with small RNAs ...
Erzsébet Kenesi +4 more
doaj +1 more source
Antagonistic and cooperative AGO2-PUM interactions in regulating mRNAs [PDF]
Scientific Reports, 2018 AbstractApproximately 1500 RNA-binding proteins (RBPs) profoundly impact mammalian cellular function by controlling distinct sets of transcripts, often using sequence-specific binding to 3′ untranslated regions (UTRs) to regulate mRNA stability and translation. Aside from their individual effects, higher-order combinatorial interactions between RBPs on
Erin L. Sternburg +4 more
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Activation of Gαq sequesters specific transcripts into Ago2 particles
Scientific Reports, 2022 AbstractThe Gαq/phospholipase Cβ1 (PLCβ1) signaling system mediates calcium responses from hormones and neurotransmitters. While PLCβ1 functions on the plasma membrane, there is an atypical cytosolic population that binds Argonaute 2 (Ago2) and other proteins associated with stress granules preventing their aggregation.
Lela Jackson +3 more
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DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes. [PDF]
PLoS ONE, 2015 Mechanistic studies of RNA processing in the RNA-Induced Silencing Complex (RISC) have been hindered by lack of methods for continuous monitoring of enzymatic activity. "Quencherless" fluorogenic substrates of RNAi enzymes enable continuous monitoring of
Mark A Bernard +2 more
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Analysis of AgoshRNA maturation and loading into Ago2
PLOS ONE, 2017 The RNA interference (RNAi) pathway was recently expanded by the discovery of multiple alternative pathways for processing of natural microRNA (miRNA) and man-made short hairpin RNA (shRNA) molecules. One non-canonical pathway bypasses Dicer cleavage and requires instead processing by Argonaute2 (Ago2), which also executes the subsequent silencing step.
Alex Harwig +4 more
openaire +6 more sources