A New Approach for Controlling Agrobacterium tumefaciens Post Transformation Using Lytic Bacteriophage [PDF]
Plants, 2022 Overgrowth of Agrobacterium tumefaciens has frequently been found in Agrobacterium-mediated plant transformation. This overgrowth can reduce transformation efficiency and even lead to explant death.
Fiqih Ramadhan +6 more
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Stable transformation mediated by Agrobacterium tumefaciens in Jonquil is better than Agrobacterium rhizogenes [PDF]
Frontiers in Plant ScienceAgrobacterium tumefaciens-mediated genetic transformation has become an important method to study gene function and create new germplasm in plants. The classic genetic transformation process is time-consuming and laborious.
Wenjie Yu +4 more
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Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment [PDF]
PlantsAuxotrophic strains of Agrobacterium tumefaciens have been developed to address the Agrobacterium overgrowth issue in plant genetic transformation; however, their application remains limited.
Lichi Zhong +3 more
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Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation [PDF]
STAR ProtocolsSummary: Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants.
Fanourios Mountourakis +2 more
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Engineering Agrobacterium tumefaciens with a Type III Secretion System to Express Type III Effectors [PDF]
Bio-Protocol, 2023 Plants elicit defense responses when exposed to pathogens, which partly contribute to the resistance of plants to Agrobacterium tumefaciens–mediated transformation.
Vidhyavathi Raman, Kirankumar Mysore
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Improved genome of Agrobacterium radiobacter type strain provides new taxonomic insight into Agrobacterium genomospecies 4 [PDF]
PeerJ, 2019 The reported Agrobacterium radiobacter DSM 30174T genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the Agrobacterium radiobacter type strain, generating a dramatically improved genome
Han Ming Gan +2 more
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Transfection in Agrobacterium tumefaciens [PDF]
Journal of Virology, 1972 Intact cells of Agrobacterium tumefaciens were examined for ability to take up biologically active LR-4 phage deoxyribonucleic acid (DNA) from the surrounding medium. DNA incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts ...
V J, Milani, G T, Heberlein
openaire +2 more sources
Frontiers in Plant Science, 2014 Agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. This ability has been utilized for plant genetic engineering. However, the efficiency is not sufficient for all plant species.
Satoko eNonaka, Hiroshi eEzura
doaj +1 more source
Susceptibility of Vicia sativa Cultivars to an Oncogenic Strain of Agrobacterium tumefaciens
Journal of Agricultural Sciences, 2003 Agrobacterium tumefaciens is commonly used in gene transfer to plants. Determination of the suitable plant genotypes facilitate this process. İn this study, eight common vetch cultivars grown in Turkey were assessed for their susceptibility to oncogenic ...
Aziz Karakaya
doaj +1 more source
Frontiers in Molecular Biosciences, 2016 Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transfor-mation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps.
Yiming Liu +8 more
doaj +1 more source