Journal of Genetic Engineering and Biotechnology, 2018 A protocol for genetic transformation mediated by Agrobacterium tumefaciens and production of transgenic Dendrobium lasianthera has been developed for the first time.
Edy Setiti Wida Utami +2 more
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A New Approach for Controlling Agrobacterium tumefaciens Post Transformation Using Lytic Bacteriophage [PDF]
Plants, 2022 Overgrowth of Agrobacterium tumefaciens has frequently been found in Agrobacterium-mediated plant transformation. This overgrowth can reduce transformation efficiency and even lead to explant death.
Fiqih Ramadhan +6 more
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Stable transformation mediated by Agrobacterium tumefaciens in Jonquil is better than Agrobacterium rhizogenes [PDF]
Frontiers in Plant ScienceAgrobacterium tumefaciens-mediated genetic transformation has become an important method to study gene function and create new germplasm in plants. The classic genetic transformation process is time-consuming and laborious.
Wenjie Yu +4 more
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Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment [PDF]
PlantsAuxotrophic strains of Agrobacterium tumefaciens have been developed to address the Agrobacterium overgrowth issue in plant genetic transformation; however, their application remains limited.
Lichi Zhong +3 more
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Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation [PDF]
STAR ProtocolsSummary: Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants.
Fanourios Mountourakis +2 more
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Engineering Agrobacterium tumefaciens with a Type III Secretion System to Express Type III Effectors [PDF]
Bio-Protocol, 2023 Plants elicit defense responses when exposed to pathogens, which partly contribute to the resistance of plants to Agrobacterium tumefaciens–mediated transformation.
Vidhyavathi Raman, Kirankumar Mysore
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DNA methylation by CcrM contributes to genome maintenance in the Agrobacterium tumefaciens plant pathogen. [PDF]
Nucleic Acids ResMartin S +7 more
europepmc +2 more sources
The Transformation and Protein Expression of the Edible Mushroom <i>Stropharia rugosoannulata</i> Protoplasts by <i>Agrobacterium-tumefaciens</i>-Mediated Transformation. [PDF]
J Fungi (Basel)Yin D, Xiong H.
europepmc +3 more sources
Improved genome of Agrobacterium radiobacter type strain provides new taxonomic insight into Agrobacterium genomospecies 4 [PDF]
PeerJ, 2019 The reported Agrobacterium radiobacter DSM 30174T genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the Agrobacterium radiobacter type strain, generating a dramatically improved genome
Han Ming Gan +2 more
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Transfection in Agrobacterium tumefaciens [PDF]
Journal of Virology, 1972 Intact cells of Agrobacterium tumefaciens were examined for ability to take up biologically active LR-4 phage deoxyribonucleic acid (DNA) from the surrounding medium. DNA incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts ...
V J, Milani, G T, Heberlein
openaire +2 more sources