A New Approach for Controlling Agrobacterium tumefaciens Post Transformation Using Lytic Bacteriophage [PDF]
Overgrowth of Agrobacterium tumefaciens has frequently been found in Agrobacterium-mediated plant transformation. This overgrowth can reduce transformation efficiency and even lead to explant death.
Fiqih Ramadhan+6 more
doaj +2 more sources
Stable transformation mediated by Agrobacterium tumefaciens in Jonquil is better than Agrobacterium rhizogenes [PDF]
Agrobacterium tumefaciens-mediated genetic transformation has become an important method to study gene function and create new germplasm in plants. The classic genetic transformation process is time-consuming and laborious.
Wenjie Yu+4 more
doaj +2 more sources
Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment [PDF]
Auxotrophic strains of Agrobacterium tumefaciens have been developed to address the Agrobacterium overgrowth issue in plant genetic transformation; however, their application remains limited.
Lichi Zhong+3 more
doaj +2 more sources
Engineering Agrobacterium tumefaciens with a Type III Secretion System to Express Type III Effectors [PDF]
Plants elicit defense responses when exposed to pathogens, which partly contribute to the resistance of plants to Agrobacterium tumefaciens–mediated transformation.
Vidhyavathi Raman, Kirankumar Mysore
doaj +2 more sources
An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host [PDF]
Agrobacterium tumefaciens is a soil-borne pathogenic bacterium that causes crown gall disease in many plants. Chemotaxis offers A. tumefaciens the ability to find its host and establish infection. Being an aerobic bacterium, A.
Zhiwei Huang+8 more
doaj +3 more sources
Improved genome of Agrobacterium radiobacter type strain provides new taxonomic insight into Agrobacterium genomospecies 4 [PDF]
The reported Agrobacterium radiobacter DSM 30174T genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the Agrobacterium radiobacter type strain, generating a dramatically improved genome
Han Ming Gan+2 more
doaj +2 more sources
A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants [PDF]
Transposition of the Anthirrinum majus Tam3 element and the Zea mays Ac element has been monitored in petunia and tobacco plants. Plant vectors were constructed with the transposable elements cloned into the leader sequence of a marker gene ...
A Day+41 more
core +14 more sources
Enhancement of Recombinant Protein Production in Transgenic Nicotiana benthamiana Plant Cell Suspension Cultures with Co-Cultivation of Agrobacterium Containing Silencing Suppressors. [PDF]
We have previously demonstrated that the inducible plant viral vector (CMViva) in transgenic plant cell cultures can significantly improve the productivity of extracellular functional recombinant human alpha-1-antiryspin (rAAT) compared with either a ...
Dandekar, Abhaya M+3 more
core +7 more sources
TagF-mediated repression of bacterial type VI secretion systems involves a direct interaction with the cytoplasmic protein Fha [PDF]
The bacterial type VI secretion system (T6SS) delivers effectors into eukaryotic host cells or toxins into bacterial competitor for survival and fitness.
Filloux, A+5 more
core +1 more source
Agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. This ability has been utilized for plant genetic engineering. However, the efficiency is not sufficient for all plant species.
Satoko eNonaka, Hiroshi eEzura
doaj +1 more source