The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T ...
Tian Li +3 more
doaj +1 more source
Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach
This study utilized a novel sampling method, ERAT (i.e. endoscopic retrograde appendicitis treatment)‐guided lumen aspiration, to obtain samples from the appendix, and shotgun metagenomic sequencing was performed for in situ characterization of the appendix microbiome in patients with acute appendicitis.
Huimin Ma +10 more
wiley +1 more source
Enhanced Virulence Gene Activity of Agrobacterium in Muskmelon (Cucumis melo L.) cv. ‘Birdie’
Muskmelon (Cucumis melo L.) cultivar ‘Birdie’, was evaluated for its response to the tumorigenic Agrobacterium tumefaciens and the oncogenic A. rhizogenes strains.
Abul K.M. MOHIUDDIN +4 more
doaj +1 more source
Plasmid required for virulence of Agrobacterium tumefaciens [PDF]
The irreversible loss of crown gall-inducing ability of Agrobacterium tumefaciens strain C-58 during growth at 37 C is shown to be due to loss of a large plasmid (1.2 X 10-8 daltons). The gene responsible for this high rate of plasmid loss at elevated temperatures seems to be located on the plasmid. In addition, another spontaneous avirulent variant, A.
Mary-Dell Chilton +4 more
openaire +3 more sources
Microneedle‐Based Biofertilizer Delivery Improves Plant Growth Through Microbiome Engineering
This figure shows how microneedles are used to deliver biofertilizers to enhance plant growth through microbial migration, metabolic reprogramming, and changes in plant endogenous microbiome. Abstract This study presents a microneedle‐based system for the delivery of rhizospheric biofertilizers into plant tissues to enhance growth.
Zhicheng Le +14 more
wiley +1 more source
Drought is one of the major constraints in durum wheat production in the Mediterranean Basin. In order to overcome this problem, the genetic transformation of durum wheat is one of the choices for improvement.
Khadija Ahansal +6 more
doaj +1 more source
Loss of PopZ At activity in Agrobacterium tumefaciens by Deletion or Depletion Leads to Multiple Growth Poles, Minicells, and Growth Defects. [PDF]
Agrobacterium tumefaciens grows by addition of peptidoglycan (PG) at one pole of the bacterium. During the cell cycle, the cell needs to maintain two different developmental programs, one at the growth pole and another at the inert old pole.
Bonnie Bassler +4 more
core +2 more sources
Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
core +1 more source
Transcriptome‐Wide Association Uncovers LncRNAs Controlling Seed Weight in Soybean
This study employs transcriptome‐wide association studies (TWAS) to identify 201 long non‐coding RNAs (lncRNAs) associated with seed weight. Expression quantitative trait locus (eQTL) analysis reveals dynamic and static regulatory variants controlling lncRNA expression across development.
Xiang Wang +8 more
wiley +1 more source
A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus. [PDF]
Agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. However, the limitations of Agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants ...
Mitsuhiro Kimura +2 more
doaj +1 more source

