Results 281 to 290 of about 21,208,579 (315)
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Aldolase enzyme gets a makeover
C&EN Global Enterprise, 2016To be used industrially, an enzyme usually needs to undergo some surgery, for instance to resculpt its active site into a shape that will accept a range of chemical substrates.
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Tetrahedron Letters, 1998
Abstract Stereoisomeric aldols 6a-d undergo retroaldolization to give 3-oxopropyl umbelliferyl ether 5 and subsequently umbelliferone 4 by β-elimination, leading to a fluorescence increase at λ em = 460 ± 20 nm ( λ ex = 360 ± 20 nm). This fluorogenic assay for aldolases operates in cell culture media and can be used to search for new ...
Nathalie Jourdain +2 more
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Abstract Stereoisomeric aldols 6a-d undergo retroaldolization to give 3-oxopropyl umbelliferyl ether 5 and subsequently umbelliferone 4 by β-elimination, leading to a fluorescence increase at λ em = 460 ± 20 nm ( λ ex = 360 ± 20 nm). This fluorogenic assay for aldolases operates in cell culture media and can be used to search for new ...
Nathalie Jourdain +2 more
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Cathepsin M: A lysosomal proteinase with aldolase-inactivating activity
Archives of Biochemistry and Biophysics, 1982Abstract A proteinase, designated cathepsin M, that catalyzes the limited modification and inactivation of fructose 1,6-bisphosphate aldolase (EC 4.1.2.13) and fructose 1,6-bisphosphatase (EC 3.1.3.11) has been partially purified from rabbit liver. On the basis of its molecular size (Mr = 30,000), activation by sulfhydryl compounds and inhibition by ...
PONTREMOLI, SANDRO +5 more
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Enzyme and Microbial Technology, 2016
Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3).
Qijia, Chen +6 more
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Sialic acid aldolases (E.C.4.1.3.3) catalyze the reversible aldol cleavage of N-acetyl-d-neuraminic acid (Neu5Ac) to from N-acetyl-d-mannosamine (ManNAc) and pyruvate. In this study, a sialic acid aldolase (PdNAL) from Peptoclostridium difficile NAP08 was expressed in Escherichia coli BL21 (DE3).
Qijia, Chen +6 more
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A non-competitive solid-phase radioimmunoassay for human aldolase A
Clinica Chimica Acta, 1982A solid-phase, non-competitive radioimmunoassay for aldolase A in human serum has been developed. Human aldolase A was purified from muscle, and specific antisera to the purified aldolase A were obtained from chickens. Specific IgG anti-human aldolase A was purified by affinity chromatography.
M, Asaka +3 more
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Bioorganic & medicinal chemistry, 1995
Three DHAP-dependent aldolases, rhamnulose-1-phosphate aldolase (Rham-1PA), fuculose-1-phosphate aldolase (Fuc-1PA) and tagatose-1,6-diphosphate aldolase (TDPA) have been cloned and overexpressed in Escherichia coli using two different expression vectors: pTrcHis for the expression of Rham-1PA and Fuc-1PA and pRSET for the expression of TDPA.
E, Garcia-Junceda +3 more
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Three DHAP-dependent aldolases, rhamnulose-1-phosphate aldolase (Rham-1PA), fuculose-1-phosphate aldolase (Fuc-1PA) and tagatose-1,6-diphosphate aldolase (TDPA) have been cloned and overexpressed in Escherichia coli using two different expression vectors: pTrcHis for the expression of Rham-1PA and Fuc-1PA and pRSET for the expression of TDPA.
E, Garcia-Junceda +3 more
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A fourth crystal form of rabbit muscle aldolase
Journal of Molecular Biology, 1972Abstract A fourth crystal form of rabbit muscle aldolase has been salted out from phosphate solutions. The space group is P6122 with a unit cell of dimensions a = 163·5 A , c= 335· 0 A , and containing 18 molecules. The possible significance of this new form is discussed briefly.
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The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK
Nature Metabolism, 2022Chen-Song Zhang, Mengqi Li, Dong-Tai Liu
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