Results 161 to 170 of about 4,402 (196)
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Bacterial alginate lyase highly active on acetylated alginates
Journal of Fermentation and Bioengineering, 1993Abstract A bacterium (strain A1) isolated from a ditch synthesized three kinds of intracellular alginate lyases: A1-I (molecular weight [M.W.] 60,000), A1-II-1 (M.W. 60,000) and A1-II-2 (M.W. 25,000) in laboratory-scale cultures. However, when cells of strain A1 were grown on an industrial scale, another lyase (A1-III) was produced other than A1-I ...
Tomohiro Hisano +7 more
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Journal of Fermentation and Bioengineering, 1991
Abstract Two kinds of bacteria producing alginate lyase were isolated from ditch sample and they were designated A-1 and A-2 strains. Morphological and physiological properties of the two strains were closely similar to each other, except that the cells of strain A-1 showed flocculation during growth on a medium containing alginate as a carbon source.
Yoshimasa Yonemoto +4 more
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Abstract Two kinds of bacteria producing alginate lyase were isolated from ditch sample and they were designated A-1 and A-2 strains. Morphological and physiological properties of the two strains were closely similar to each other, except that the cells of strain A-1 showed flocculation during growth on a medium containing alginate as a carbon source.
Yoshimasa Yonemoto +4 more
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Kinetics and specificity of alginate lyases
Hydrobiologia, 1993A purified preparation of the extracellular alginate lyase has been used to study kinetics and specificity towards purified, homopolymeric fragments of alginate. The enzyme preparation from Bacillus circulans 1351 degraded both block types, although with different efficiency, and thus appears to be nonspecific.
Bj�rn Larsen +2 more
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Characterization of Alginate Lyase Activity on Liquid, Gelled, and Complexed States of Alginate
Biotechnology Progress, 2007A study of alginate lyase was carried out to determine if this enzyme could be used to remove alginate present in the core of alginate/poly-L-lysine (AG/PLL) microcapsules in order to maximize cell growth and colonization. A complete kinetic study was undertaken, which indicated an optimal activity of the enzyme at pH 7-8, 50 degrees C, in the presence
Breguet V, von Stockar U, Marison IW
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Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase
Applied Microbiology and Biotechnology, 2003Alginate is an industrially relevant linear copolymer composed of beta-1,4-linked D-mannuronic acid and its C-5 epimer L-guluronic acid. The rheological and gel-forming properties of alginates depend on the molecular weight and the relative content of the two monomers. Alginate produced by Azotobacter vinelandii was shown to be degraded towards the end
M A, Trujillo-Roldán +4 more
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World Journal of Microbiology and Biotechnology, 2007
The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
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The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
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Bacterial alginate lyase inactive on alginate biosynthesized by Pseudomonas aeruginosa
Journal of Fermentation and Bioengineering, 1993A bacterium (strain Al) isolated from a ditch produces three kinds of intracellular alginate lyases [Al-I (molecular weight: M.W. 60,000), Al-II-1 (M.W. 60,000) and Al-II-2]; the former two lyases have been purified and characterized (Yonemoto et al., J. Ferment. Bioeng., 72, 152–157, 1991).
Tomohiro Hisano +7 more
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Isolation of an alginate-degrading organism and purification of its alginate lyase
Journal of Fermentation and Bioengineering, 1991Abstract We isolated a bacterial strain, OS-ALG-9, which solubilized immobilized gel with calcium alginate in a reactor. It was identified as Pseudomonas sp. We studied the cultural conditions by using a 2.5- l jar fermentor and found that the growth and alginate-degrading enzyme production were optimal at pH 7.0 and 30°C.
Shinichi Kinoshita +4 more
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Alginate lyase: Structure, property, and application
Biotechnology and Bioprocess Engineering, 2011Alginate is a linear polysaccharide in which β-D-mannuronate (M) and its epimer, α-L-guluronate (G), are covalently (1–4)-linked in different sequences. Alginate is mainly used as a food additive to modify food texture due to its high viscosity and gelling property.
Hee Sook Kim +2 more
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2019
With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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