Expression, Purification, and Characterisation of Recombinant Alginate Lyase (Flammeovirga AL2) for the Bioconversion of Alginate into Alginate Oligosaccharides [PDF]
MoleculesAlginate, a polysaccharide found in brown seaweeds, has regularly gained attention for its potential use as a source of bioactive compounds. However, it is structurally complex with a high molecular weight, limiting its application.
Coleen E. Grobler +4 more
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A Novel Alginate Lyase: Identification, Characterization, and Potential Application in Alginate Trisaccharide Preparation. [PDF]
Mar Drugs, 2022 Alginate oligosaccharides (AOS) have many biological activities and significant applications in prebiotics, nutritional supplements, and plant growth development. Alginate lyases have unique advantages in the preparation of AOS. However, only a limited number of alginate lyases have been so far reported to have potentials in the preparation of AOS with
Xue Z +7 more
europepmc +7 more sources
Characterization of AlgMsp, an alginate lyase from Microbulbifer sp. 6532A.
PLoS ONE, 2014 Alginate is a polysaccharide produced by certain seaweeds and bacteria that consists of mannuronic acid and guluronic acid residues. Seaweed alginate is used in food and industrial chemical processes, while the biosynthesis of bacterial alginate is ...
Steven M Swift +4 more
doaj +3 more sources
Genome Analysis of a Potential Novel Vibrio Species Secreting pH- and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides [PDF]
Marine DrugsAlginate lyase is an attractive biocatalyst that can specifically degrade alginate to produce oligosaccharides, showing great potential for industrial and medicinal applications.
Ke Bao +4 more
doaj +2 more sources
Cloning and Characterization of a Novel Alginate Lyase from Paenibacillus sp. LJ-23 [PDF]
Marine Drugs, 2022 As a low molecular weight alginate, alginate oligosaccharides (AOS) exhibit improved water solubility, better bioavailability, and comprehensive health benefits. In addition, their biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and
Mingpeng Wang +6 more
doaj +2 more sources
A bifunctional endolytic alginate lyase with two different lyase catalytic domains from Vibrio sp. H204 [PDF]
Frontiers in MicrobiologyAlginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by β-elimination. Here, we identified the bifunctional endolytic alginate lyase Aly35 from the marine bacterium Vibrio sp. Strain H204.
Chune Peng +9 more
doaj +2 more sources
High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide [PDF]
Marine Drugs, 2018 An alginate lyase encoding gene sagl from Flavobacterium sp. H63 was codon optimized and recombinantly expressed at high level in P.pastoris through high cell-density fermentation.
Haifeng Li +3 more
doaj +2 more sources
Production of Alginate Oligosaccharides (AOSs) Using Enhanced Physicochemical Properties of Immobilized Alginate Lyase for Industrial Application [PDF]
Marine DrugsAlginate lyase (AL) is a polysaccharide-degrading enzyme that can degrade alginate by hydrolyzing glycosidic bonds and produces unsaturated alginate oligosaccharides (AOSs). These AOSs have wide therapeutic and nutraceutical applications.
Simranjeet Kaur +3 more
doaj +2 more sources
Characterization of a New Bifunctional and Cold-Adapted Polysaccharide Lyase (PL) Family 7 Alginate Lyase from Flavobacterium sp. [PDF]
Marine Drugs, 2020 Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding gene alyS02 from Flavobacterium sp. S02 was recombinantly expressed at a high level
Hai-Xiang Zhou +4 more
doaj +2 more sources
Sustainable alginate lyases catalyzed degradation of bio-based carbohydrates
Frontiers in Chemistry, 2022 Alginate is a water-soluble and acidic polysaccharide derived from the cell wall and intercellular substance of brown algae. It is widely distributed in brown algae, such as Laminaria, Sargassum, and Macrocystis, etc.
Zhiguo Zheng +3 more
doaj +3 more sources