Results 31 to 40 of about 4,402 (196)

Alginate Lyases from Alginate-Degrading Vibrio splendidus 12B01 Are Endolytic [PDF]

open access: yesApplied and Environmental Microbiology, 2015
ABSTRACT Alginate lyases are enzymes that degrade alginate through β-elimination of the glycosidic bond into smaller oligomers. We investigated the alginate lyases from Vibrio splendidus 12B01, a marine bacterioplankton species that can grow on alginate as its sole carbon source.
Ahmet H, Badur   +5 more
openaire   +2 more sources

Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate [PDF]

open access: yes, 1993
peer reviewedThe recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872).
Malissard, Martine   +8 more
core   +1 more source

Preparation of Dye-labeled Alginate for the Assay of Alginate Lyase

open access: yesBioscience, Biotechnology, and Biochemistry, 1997
Dabsyl-alginate, the dye-labeled substrate of alginate lyase, was prepared. Low-viscosity alginate, which was prepared by enzymatic degradation of sodium alginate, and tetramethylenediamine were conjugated by reductive amination. Then, dabsyl-Cl was coupled with the primary amino group of the aminobutyl-alginate. The assay for endo-alginate lyase using
YOSHIDA, Shigeki   +4 more
openaire   +1 more source

Role of an Alginate Lyase for Alginate Transport in Mucoid Pseudomonas aeruginosa [PDF]

open access: yesInfection and Immunity, 2005
ABSTRACT The opportunistic pathogen Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate that is important for evasion of host defenses, especially during chronic pulmonary disease of patients with cystic fibrosis (CF).
Sumita, Jain, Dennis E, Ohman
openaire   +2 more sources

Data_Sheet_1_The Function of CBM32 in Alginate Lyase VxAly7B on the Activity on Both Soluble Sodium Alginate and Alginate Gel.docx

open access: yes, 2022
Carbohydrate-binding modules (CBMs), as an important auxiliary module, play a key role in degrading soluble alginate by alginate lyase, but the function on alginate gel has not been elucidated.
Shan Gao (46743)   +7 more
core   +1 more source

Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX [PDF]

open access: yesJournal of Bacteriology, 1996
Previous studies localized an alginate lyase gene (algL) within the alginate biosynthetic gene cluster at 34 min on the Pseudomonas aeruginosa chromosome. Insertion of a Tn501 polar transposon in a gene (algX) directly upstream of algL in mucoid P. aeruginosa FRD1 inactivated expression of algX, algL, and other downstream genes, including algA.
S R, Monday, N L, Schiller
openaire   +2 more sources

Alginate Lyase of a Novel Algae Fermentation Strain [PDF]

open access: yes, 2019
A novel algae fermentation strain was obtained in our previous work. This strain can produce alginate lyase and alcohol dehydrogenase used for the ethanol fermentation from algae.
X. Xia   +5 more
core   +1 more source

Properties of Alginate Lyases from Marine Bacteria [PDF]

open access: yesApplied and Environmental Microbiology, 1984
Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved ...
R S, Doubet, R S, Quatrano
openaire   +2 more sources

Merging Biocatalysis and Chemocatalysis in Flow: State‐of‐the‐Art and Future Directions for Sustainable Synthesis

open access: yesAngewandte Chemie, EarlyView.
This review highlights recent advances in integrating biocatalysis and chemocatalysis in continuous flow to create streamlined, sustainable processes. It examines chemo‐enzymatic cascades combining at least one enzymatic and one chemical step, discusses challenges such as enzyme immobilization, leaching, and reactor clogging, and presents solutions ...
Petros Siasiaridis   +2 more
wiley   +2 more sources

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