Marine Biotechnology, 2009 The alginate lyase structural gene (AlyVI gene) was amplified from plasmid pET24-ALYVI carrying the alginate lyase gene from the marine bacterium Vibrio sp. QY101 which is a pathogen of Laminaria sp. When the gene was cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica, the
Liu, Guanglei +5 more
openaire +4 more sources
Characterization of AlgMsp, an alginate lyase from Microbulbifer sp. 6532A.
PLoS ONE, 2014 Alginate is a polysaccharide produced by certain seaweeds and bacteria that consists of mannuronic acid and guluronic acid residues. Seaweed alginate is used in food and industrial chemical processes, while the biosynthesis of bacterial alginate is ...
Steven M Swift +4 more
doaj +2 more sources
Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate [PDF]
Squalen, 2017 Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity.
Subaryono Subaryono +4 more
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An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall [PDF]
Scientific Reports, 2021 In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting ...
Mónica Costa +8 more
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Marine Drugs, 2020 Alginate extracted from widely cultured brown seaweed can be hydrolyzed by alginate lyase to produce alginate oligosaccharides (AOS) with intriguing biological activities.
Yong-Hui Zhang +5 more
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Marine Drugs, 2020 Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding gene alyS02 from Flavobacterium sp. S02 was recombinantly expressed at a high level
Hai-Xiang Zhou +4 more
doaj +2 more sources
Synergy of the Two Alginate Lyase Domains of a Novel Alginate Lyase from Vibrio sp. NC2 in Alginate Degradation. [PDF]
Appl Environ Microbiol, 2022 Alginate is a major component in the cell walls of brown algae. Alginate degradation is carried out by alginate lyases.
Wang XH +6 more
europepmc +3 more sources
Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property
Marine Drugs, 2019 Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene,
Yanan Wang +8 more
doaj +2 more sources
Evolving Strategies for Marine Enzyme Engineering: Recent Advances on the Molecular Modification of Alginate Lyase [PDF]
Marine Life Science and Technology, 2022 Benwei Zhu, Shengsheng Cao, Limin Ning
exaly +2 more sources
Overexpression of Thermal and pH Stable Alginate Lyase of P. aeruginosa 293 and In silico Study of algL Gene [PDF]
Journal of Genetic Resources, 2021 P. aeruginosa is an opportunistic bacterium that produces a capsule-like polysaccharide called alginate in response to various stimuli. The mucoid strain of Pseudomonas aeruginosa produces alginate which is an exopolysaccharide and is involved in the ...
Maryam Zali Benekohal +4 more
doaj +1 more source