Results 11 to 20 of about 4,402 (196)

Expression, Purification, and Characterisation of Recombinant Alginate Lyase (Flammeovirga AL2) for the Bioconversion of Alginate into Alginate Oligosaccharides [PDF]

Molecules
Alginate, a polysaccharide found in brown seaweeds, has regularly gained attention for its potential use as a source of bioactive compounds. However, it is structurally complex with a high molecular weight, limiting its application.
Blessing Mabate, Marilize Le Roes-Hill, Brett I. Pletschke, Grobler, Coleen E., Mabate, Blessing, Le Roes-Hill, Marilize, Coleen E. Grobler, Prins, Alaric, Alaric Prins, Pletschke, Brett I.   +9 more
core   +5 more sources

The Function of CBM32 in Alginate Lyase VxAly7B on the Activity on Both Soluble Sodium Alginate and Alginate Gel [PDF]

Frontiers in Microbiology, 2022
Carbohydrate-binding modules (CBMs), as an important auxiliary module, play a key role in degrading soluble alginate by alginate lyase, but the function on alginate gel has not been elucidated. Recently, we reported alginate lyase VxAly7B containing a CBM32 and a polysaccharide lyase family 7 (PL7).
Luyao Tang, Luyao Tang, Luyao Tang, Luyao Tang, Enwen Guo, Enwen Guo, Enwen Guo, Enwen Guo, Lan Zhang, Lan Zhang, Lan Zhang, Lan Zhang, Ying Wang, Ying Wang, Ying Wang, Ying Wang, Shan Gao, Shan Gao, Shan Gao, Shan Gao, Mengmeng Bao, Mengmeng Bao, Mengmeng Bao, Mengmeng Bao, Feng Han, Feng Han, Feng Han, Feng Han, Wengong Yu, Wengong Yu, Wengong Yu, Wengong Yu   +31 more
openaire   +4 more sources

A Novel Cold-Adapted and High-Alkaline Alginate Lyase with Potential for Alginate Oligosaccharides Preparation [PDF]

Molecules, 2023
Alginate oligosaccharides (AOs) prepared through enzymatic reaction by diverse alginate lyases under relatively controllable and moderate conditions possess versatile biological activities. But widely used commercial alginate lyases are still rather rare
Hui-Wen Lei, Hai-Hua Cong, Zhi-Fang Chen, Zhi-Hong Zheng, Hai-Xiang Zhou, Hai-Ying Wang   +5 more
core   +2 more sources

A bifunctional endolytic alginate lyase with two different lyase catalytic domains from Vibrio sp. H204 [PDF]

Front Microbiol
Alginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by β-elimination. Here, we identified the bifunctional endolytic alginate lyase Aly35 from the marine bacterium Vibrio sp. Strain H204.
Xinkun Wang, Qingbin Wang, Qianqian Zheng, Wei Xu, Fuchuan Li, Chune Peng, Xiaodan Dong, Lizeng Peng, Xiaohui Liang   +8 more
core   +2 more sources

Production of Alginate Oligosaccharides (AOSs) Using Enhanced Physicochemical Properties of Immobilized Alginate Lyase for Industrial Application [PDF]

Mar Drugs
Alginate lyase (AL) is a polysaccharide-degrading enzyme that can degrade alginate by hydrolyzing glycosidic bonds and produces unsaturated alginate oligosaccharides (AOSs). These AOSs have wide therapeutic and nutraceutical applications.
Simranjeet Kaur, Reinu E. Abraham, Christopher M. M. Franco, Munish Puri   +3 more
core   +3 more sources

Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524

Marine Drugs, 2011
An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp.
Sheng Dong, Xiu-Lan Chen, Yu-Zhong Zhang   +2 more
exaly   +2 more sources

Purification and characterization of a novel alginate lyase from a marine streptomyces species isolated from seaweed [PDF]

Mar Drugs, 2021
Alginate, a natural polysaccharide derived from brown seaweed, is finding multiple applications in biomedicine via its transformation through chemical, physical, and, increasingly, enzymatic processes.
Franco, CMM, Nguyen, TNT, Araujo, R, Chataway, T, Puri, M   +4 more
core   +2 more sources

Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase [PDF]

Applied and Environmental Microbiology, 2021
The potential markets for seaweed-derived products and seaweed processing technologies are growing, yet commercial enzyme cocktails for complete conversion of seaweed to fermentable sugars are not available. Such an enzyme cocktail would require the catalytic properties of a variety of different enzymes, where fucoidanases, laminarinases, and ...
Magnus Ø. Arntzen, Bjørn Pedersen, Leesa J. Klau, Runar Stokke, Maren Oftebro, Simen G. Antonsen, Lasse Fredriksen, Håvard Sletta, Olav A. Aarstad, Finn L. Aachmann, Svein J. Horn, Vincent G. H. Eijsink   +11 more
openaire   +4 more sources

Purification and properties of an alginate lyase from a marine bacterium [PDF]

Biochemical Journal, 1976
An unidentified pseudomonad isolated by enrichment procedures from decomposing seaweed was grown in defined medium containing sodium alginate as the sole carbon source. The alginate lyase recovered from disrupted bacterial cells was purified by a procedure of (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography.
I. W. Davidson, Ian W. Sutherland, C. J. Lawson   +2 more
openalex   +4 more sources

High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide [PDF]

, 2018
An alginate lyase encoding gene sagl from Flavobacterium sp. H63 was codon optimized and recombinantly expressed at high level in P.pastoris through high cell-density fermentation.
Liehuan Chen, Shuling Wang, Haifeng Li, Yunyi Zhang   +3 more
core   +2 more sources