Alginate Lyase (AlgL) Activity Is Required for Alginate Biosynthesis in Pseudomonas aeruginosa [PDF]
ABSTRACT To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa , an algLΔ ::Gm r mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but
Mark T, Albrecht, Neal L, Schiller
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Bacteriophage-encoded depolymerases: their diversity and biotechnological applications [PDF]
Bacteriophages (phages), natural enemies of bacteria, can encode enzymes able to degrade polymeric substances. These substances can be found in the bacterial cell surface, such as polysaccharides, or are produced by bacteria when they are living in ...
A Abdi-Ali +112 more
core +1 more source
Functional Exploration of the Polysaccharide Lyase Family PL6. [PDF]
Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues).
Sophie Mathieu +4 more
doaj +1 more source
Impact of different alginate lyases on combined cellulase–lyase saccharification of brown seaweed [PDF]
Alginate attack characteristics and impact on cellulase–lyase catalyzed saccharification of brown seaweed were compared for three microbial PL7 alginate lyases (EC 4.2.2.-) two of them heterologously expressed in Escherichia coli as part of the work.
Manns, Dirk Martin +3 more
core +1 more source
Alginate Lyases: Sources, Mechanism of Activity and Potencial Application
Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological ...
Subaryono Subaryono +3 more
doaj +1 more source
Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX [PDF]
Previous studies localized an alginate lyase gene (algL) within the alginate biosynthetic gene cluster at 34 min on the Pseudomonas aeruginosa chromosome. Insertion of a Tn501 polar transposon in a gene (algX) directly upstream of algL in mucoid P. aeruginosa FRD1 inactivated expression of algX, algL, and other downstream genes, including algA.
S R, Monday, N L, Schiller
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Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process [PDF]
Bacteriophages are bacterial viruses that infect the host after successful receptor recognition and adsorption to the cell surface. The irreversible adherence followed by genome material ejection into host cell cytoplasm must be preceded by the passage ...
Briers, Yves +4 more
core +1 more source
Objective: This study aimed to screen a strain with high alginate lyase activity, identify it, optimize the fermentation conditions, and evaluate the degradation effect on Sargassum of the strain.
Shuang LUO +8 more
doaj +1 more source
Co-axial wet-spinning in 3D Bioprinting: state of the art and future perspective of microfluidic integration [PDF]
Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions ...
Barbetta, Andrea +3 more
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Expression and Characterization of an Alginate Lyase and Its Thermostable Mutant in Pichia pastoris
Alginate is one of the most abundant polysaccharides in algae. Alginate lyase degrades alginate through a β-elimination mechanism to produce alginate oligosaccharides with special bioactivities. Improving enzyme activity and thermal stability can promote
Suxiao Yang +6 more
doaj +1 more source

