Results 71 to 80 of about 3,190 (162)

Characterization of a New Bifunctional and Cold-Adapted Polysaccharide Lyase (PL) Family 7 Alginate Lyase from Flavobacterium sp.

open access: yesMarine Drugs, 2020
Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding gene alyS02 from Flavobacterium sp. S02 was recombinantly expressed at a high level
Hai-Xiang Zhou   +4 more
doaj   +1 more source

Living Microbial Drugs

open access: yesChemistry – A European Journal, Volume 32, Issue 26, 11 July 2026.
The introduction outlines the review scope. Microbial cell factories as living drugs cover host–gut microbiota, bacteria, yeast, and other microbial systems, with comparative host advantages. Engineering strategies include synthetic circuits, quorum sensing, and memory.
Cemile Elif Özçelik   +3 more
wiley   +1 more source

Aqueous Two‐Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells

open access: yesAdvanced Healthcare Materials, Volume 15, Issue 25, 3 July 2026.
Aqueous two‐phase systems (ATPS) enable the formation of biomimetic interfaces crucial for tissue engineering. However, clinical translation remains limited by the challenge of precisely controlling cellular compartmentalization. Here, we developed ATPS biomaterial inks for 3D bioprinting allowing tuneable droplet formation via NaCl modulation.
Martina Marcotulli   +17 more
wiley   +1 more source

A Versatile Microfluidic Extrusion‐Based Hydrogel Platform for Self‐Organization and Long‐Term Maintenance of Engineered 3D Lymphatic Endothelium

open access: yesAdvanced Healthcare Materials, Volume 15, Issue 25, 3 July 2026.
Microfluidic coaxial extrusion generates size‐controlled 3D lymphatic tubes from primary human dermal lymphatic endothelial cells in a defined four‐component matrix. These engineered vessels self‐organize into stable lymphatic endothelium, maintain selective macromolecular permeability for 30 days, and enable direct comparison with blood endothelial ...
Elsa Mazari‐Arrighi   +8 more
wiley   +1 more source

Alliinase: A Comprehensive Review of the Pivotal Enzyme in Garlic Chemistry and Its Applications

open access: yesFood Frontiers, Volume 7, Issue 4, July 2026.
Garlic crude extract was purified to obtain alliinase, which catalyzes alliin hydrolysis to release allicin. The free and immobilized alliinase shows potential applications in food, biotechnology, pharmaceuticals, health products, and biosensors. ABSTRACT Alliinase is a pyridoxal 5′‐phosphate (PLP)‐dependent lyase that serves as the cornerstone of the ...
Zhenlin Han   +5 more
wiley   +1 more source

High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide

open access: yesMarine Drugs, 2018
An alginate lyase encoding gene sagl from Flavobacterium sp. H63 was codon optimized and recombinantly expressed at high level in P.pastoris through high cell-density fermentation.
Haifeng Li   +3 more
doaj   +1 more source

Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

open access: yesBiotechnology for Biofuels, 2020
Background As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because the unsaturated monosaccharides ...
Luyao Tang   +6 more
doaj   +1 more source

Antioxidants in Plant‐Based Food Matrices: From Structure–Activity and Degradation Kinetics to Formulation Design

open access: yesComprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 4, July 2026.
ABSTRACT Plant‐based antioxidants are widely incorporated into foods to retard oxidative deterioration and to deliver health‐related benefits. Yet, their in‐product and in vivo performance frequently diverges from predictions based on solution‐phase chemical assays, because matrix interactions, processing history, and host metabolism reshape both ...
Márcio Vargas‐Ramella   +6 more
wiley   +1 more source

Enzymatic preparation of alginate oligosaccharides and its application in aquaculture

open access: yesSiliao yanjiu
Alginate oligosaccharides are a kind of low molecular weight polymer composed of 2~10 monosaccharide molecules linked by the same or different glycosidic bonds. Alginate lyase is an important tool for the enzymatic preparation of AOS. AOS prepared by the
HUANG Zhe   +6 more
doaj  

Marine Bacteria, A Source for Alginolytic Enzyme to Disrupt Pseudomonas aeruginosa Biofilms

open access: yesMarine Drugs, 2019
Pseudomonas aeruginosa biofilms are typically associated with the chronic lung infection of cystic fibrosis (CF) patients and represent a major challenge for treatment. This opportunistic bacterial pathogen secretes alginate, a polysaccharide that is one
Said M. Daboor   +4 more
doaj   +1 more source

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