Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]
, 2015 Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F +5 more
core +1 more source
Animals, 2021 The bovine lymphocyte antigen (BoLA-DRB3) gene is an important region that codes for glycoproteins responsible for the initiation of an immune response.
Lwamkelekile Sitshilelo Mkize +1 more
doaj +1 more source
Zhongguo shuxue zazhi, 2022 Objective To investigate the association between HLA-A, B, DRB1 alleles and chronic renal failure (CRF) in Han population of Shandong Peninsula. Methods Sequence specific oligonucleotide probe-polymerase chain reaction (PCR-SSO) was used to genotype 880 ...
Rong WANG +5 more
doaj +1 more source
Does the catechol-O-methyltransferase (COMT) Val158Met human polymorphism in influence procrastination? [PDF]
, 2017 Genetic studies are enlightening how the expression of several genes influences neuronal activity and all facets of human normal and abnormal behaviour. Among these, a growing body of information shows that a few key genes regulating activity of central ...
Abate, Georgia +3 more
core +1 more source
Interactive Effects of Physical Activity and APOE-ε4 On White Matter Tract Diffusivity in Healthy Elders [PDF]
, 2016 Older adult apolipoprotein-E epsilon 4 (APOE-ε4) allele carriers vary considerably in the expression of clinical symptoms of Alzheimer\u27s disease (AD), suggesting that lifestyle or other factors may offer protection from AD-related neurodegeneration ...
Durgerian, Sally +7 more
core +2 more sources
Characterization of Bordetella pertussis Strains Isolated from India
Pathogens, 2022 Despite high level vaccination and the availability of two different types of vaccines, whole cell (wP) and acellular vaccines (aP), the resurgence of pertussis has been reported in many countries.
Shweta Alai +5 more
doaj +1 more source
A functional variant in the serotonin receptor 7 gene (HTR7), rs7905446, is associated with good response to SSRIs in bipolar and unipolar depression. [PDF]
, 2020 Predicting antidepressant response has been a clinical challenge for mood disorder. Although several genome-wide association studies have suggested a number of genetic variants to be associated with antidepressant response, the sample sizes are small and
Aitchison, Katherine J +14 more
core +2 more sources
Delimiting Allelic Imbalance of TYMS by Allele-Specific Analysis
Medicine, 2015 Allelic imbalance of thymidylate synthase (TYMS) is attributed to polymorphisms in the 5'- and 3'-untranslated region (UTR). These polymorphisms have been related to the risk of suffering different cancers, for example leukemia, breast or gastric cancer, and response to different drugs, among which are methotrexate glutamates, stavudine, and ...
Carracedo Álvarez, Ángel +3 more
openaire +3 more sources
Bayesian inference of natural selection from allele frequency time series
, 2016 The advent of accessible ancient DNA technology now allows the direct ascertainment of allele frequencies in ancestral populations, thereby enabling the use of allele frequency time series to detect and estimate natural selection.
Evans, Steven N. +2 more
core +1 more source
Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. [PDF]
, 2017 With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public ...
Carvajal-Carmona, Luis G +4 more
core +4 more sources