Results 71 to 80 of about 63,430 (242)

Nanodroplet Array Platform for Integrated Synthesis and Screening of MEK Inhibitors: a Miniaturized Approach to Early Drug Discovery

Angewandte Chemie International Edition, EarlyView.
We present a nanodroplet array platform that integrates solid‐phase synthesis, MALDI‐MS analysis, and cell‐based screening. Using nanoliter droplets, we synthesized and tested 325 potential MEK inhibitors and identified 46 active compounds. This miniaturized approach combines chemistry and biology on a single chip, reducing time, cost, and material use
Maximilian Seifermann, Julius Höpfner, Liana Bauer, Divya Varadharajan, Stefan Schmidt, Björn Fröhlich, Benjamin Wellenhofer, Charlotte Luchena, Carsten Hopf, Anna A. Popova, Pavel A. Levkin   +10 more
wiley   +1 more source

Structural basis for the allosteric regulation and dynamic assembly of DNMT3B. [PDF]

Nucleic Acids Res, 2023
Lu J, Fang J, Zhu H, Liang KL, Khudaverdyan N, Song J.   +5 more
europepmc   +1 more source

Biochemical mechanism of uracil uptake regulation in Escherichia coli B. Allosteric effects on uracil phosphoribosyltransferase under stringent conditions.

, 1977
R Fast, Ola Sköld
openalex   +1 more source

Expanding the Repertoire of Photoswitchable Unnatural Amino Acids for Enzyme Engineering

Angewandte Chemie International Edition, EarlyView.
Enzyme engineering with photoswitchable unnatural amino acids (psUAAs) has a high potential for applications such as biocatalysis. Here, we extend the current repertoire of psUAAs to include more versatile properties paving the way to more advanced photocontrol engineering.
Caroline Hiefinger, Michela Marcon, Verena Pape, Guillem Casadevall, Ranit Lahmy, Christoph Haag, Julian Nazet, Michael Bartl, Astrid Bruckmann, Sílvia Osuna, Burkhard König, Andrea Hupfeld   +11 more
wiley   +1 more source

Biophysical Mechanism of Allosteric Regulation of Actin Capping Protein. [PDF]

J Mol Biol, 2023
Mooren OL, Stuchell-Brereton MD, McConnell P, Yan C, Wilkerson EM, Goldfarb D, Cooper JA, Sept D, Soranno A.   +8 more
europepmc   +1 more source

Allosteric transitions of phosphorylase a and the regulation of glycogen metabolism.

, 1978
Neil B. Madsen, Peter J. Kasvinsky, Robert J. Fletterick   +2 more
openalex   +1 more source

Allosteric Regulation of Catalytic Activity: Escherichia coli Aspartate Transcarbamoylase versus Yeast Chorismate Mutase [PDF]

, 2001
Kerstin Helmstaedt, Sven Krappmann, Gerhard H. Braus   +2 more
openalex   +1 more source

Activation of a Secondary‐Messenger Receptor via Allosteric Modulation of a Dynamic Conformational Ensemble

Angewandte Chemie International Edition, EarlyView.
Intrinsically disordered loops within globular protein domains have long escaped structural biology. Here, Söldner et al. show that despite this disorder, an allosteric modulation is exerted in a secondary‐messenger receptor by binding of its secondary messenger.
Benedikt Söldner, Himanshu Singh, Elias Akoury, Gregor Witte, Rasmus Linser   +4 more
wiley   +1 more source

The gdhB Gene of Pseudomonas aeruginosa Encodes an Arginine-Inducible NAD ⁺ -Dependent Glutamate Dehydrogenase Which Is Subject to Allosteric Regulation [PDF]

, 2001
Chung‐Dar Lu, Ahmed T. Abdelal
openalex   +1 more source

Monovalent Cation Effects on Lysine-sensitive Aspartokinase Catalytic Activity and Allosteric Regulation

, 1974
Jane D. Funkhouser, W. Smith
openalex   +1 more source