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d-Allulose enhances postprandial fat oxidation in healthy humans

Nutrition, 2017
d-Allulose, a C-3 epimer of d-fructose, has been reported to decrease body weight and adipose tissue weight in animal studies and is expected to be a potent antiobese sweetener. Our animal study suggested that one of the mechanisms of d-allulose's antiobesity function is an increase in energy expenditure.
Tomonori, Kimura   +6 more
openaire   +2 more sources

Tailored magnetic silica-immobilized D-allulose 3-epimerase with enhanced stability and recyclability for efficient D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie functional sweetener, is produced by the enzymatic conversion of d-fructose via D-allulose 3-epimerase (DAE) and holds significant market potential, particularly for individuals with obesity and diabetes. However, the limited reusability and stability of DAE have restricted its industrial application.
Ziqun, Xiao   +4 more
openaire   +2 more sources

Advances in the biosynthesis of D-allulose

World Journal of Microbiology and Biotechnology
D-allulose is a rare monosaccharide and a C-3 epimer of D-fructose. It has physiological functions, such as antihyperglycemic, obesity-preventing, neuroprotective, and reactive oxygen species (ROS) scavenging effects, making it an ideal sugar substitute. The synthesis methods for D-allulose include chemical synthesis and biosynthesis.
Yue, Zhang   +9 more
openaire   +2 more sources

D-allulose, a versatile rare sugar: recent biotechnological advances and challenges

Critical Reviews in Food Science and Nutrition, 2021
D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g dietary energy, and special physiological functions. It has been approved as GRAS by the U.S. Food and Drug Administration, and is allowed to be excluded from total and
Wenli Zhang   +7 more
openaire   +2 more sources

Insulin mimetic effect of D‐allulose on apolipoprotein A‐I gene

Journal of Food Biochemistry, 2022
Several nutrients modulate the transcriptional activity of the apolipoprotein A-I (apo A-I) gene. To determine the influence of rare sugars on apo A-I expression in hepatic (HepG2) and intestinal derived (Caco-2) cell lines, apo A-I, albumin, and SP1 were quantified with enzyme immunoassay and Western blots while mRNA levels were quantified with real ...
Michael J. Haas   +7 more
openaire   +2 more sources

Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Journal of Biotechnology
D-allulose, a naturally occurring monosaccharide, is present in small quantities in nature. It is considered a valuable low-calorie sweetener due to its low absorption in the digestive tract and zero energy for growth. Most of the recent efforts to produce D-allulose have focused on in vitro enzyme catalysis. However, microbial fermentation is emerging
Qiang, Guo   +5 more
openaire   +2 more sources

A study of D-allulose-associated reproductive toxicity in rats

Food and Chemical Toxicology, 2019
In this study, we assessed whether D-allulose was associated with reproductive toxicity in rats, assessing reproduction and offspring growth following gavage of parents with 0, 500, 1000, and 2000 mg/kg of this compound. Specifically, female rats were continuously dose from 2 weeks prior to mating until day 21 of lactation, while males were dose for ...
Hyejung, Kim   +7 more
openaire   +2 more sources

Designable immobilization of D-allulose 3-epimerase on bimetallic organic frameworks based on metal ion compatibility for enhanced D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang   +4 more
openaire   +2 more sources

Elucidation of d-allulose recognition mechanism in ketose 3-epimerase

Journal of Bioscience and Bioengineering
d-Allulose is a low-calorie sweetener with multiple nutritional functions that can be produced through d-fructose isomerization by ketose 3-epimerase (KEase). l-Ribulose 3-epimerase from Arthrobacterglobiformis (AgLRE) is one of the most important enzymes that produce d-allulose; however, its substrate recognition mechanism is unknown.
Masahiro Watanabe   +2 more
openaire   +2 more sources

Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Bioprocess and Biosystems Engineering
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening.
Ji-Dong, Shen   +8 more
openaire   +2 more sources

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