Improved alpharetrovirus-based Gag.MS2 particles for efficient and transient delivery of CRISPR-Cas9 into target cells [PDF]
Molecular Therapy - Nucleic Acids, 2022 DNA-modifying technologies, such as the CRISPR-Cas9 system, are promising tools in the field of gene and cell therapies. However, high and prolonged expression of DNA-modifying enzymes may cause cytotoxic and genotoxic side effects and is therefore unwanted in therapeutic approaches.
Dirk Hoffmann +2 more
exaly +4 more sources
Inhibition of Alpharetrovirus Replication by a Range of Human APOBEC3 Proteins [PDF]
Journal of Virology, 2007 ABSTRACT The mammalian APOBEC3 family of cytidine deaminases includes members that can act as potent inhibitors of retroviral infectivity and retrotransposon mobility. Here, we have examined whether the alpharetrovirus Rous sarcoma virus (RSV) is susceptible to inhibition by a range of human APOBEC3 proteins.
Heather L Wiegand, Bryan R Cullen
exaly +3 more sources
Integrated multi-omics identified the novel intratumor microbiome-derived subtypes and signature to predict the outcome, tumor microenvironment heterogeneity, and immunotherapy response for pancreatic cancer patients. [PDF]
Front Pharmacol, 2023 Background: The extremely malignant tumour known as pancreatic cancer (PC) lacks efficient prognostic markers and treatment strategies. The microbiome is crucial to how cancer develops and responds to treatment. Our study was conducted in order to better
Zhang B +8 more
europepmc +3 more sources
A Structural Perspective of the Role of IP6 in Immature and Mature Retroviral Assembly. [PDF]
Viruses, 2021 The small cellular molecule inositol hexakisphosphate (IP6) has been known for ~20 years to promote the in vitro assembly of HIV-1 into immature virus-like particles.
Obr M, Schur FKM, Dick RA.
europepmc +4 more sources
Transcriptome mining reveals diversity and evolution of circulating and endogenous amphibian retroviruses. [PDF]
RetrovirologyBackground: The evolutionary history of retroviruses and their impact on vertebrate evolution remains poorly understood, particularly in non-mammalian hosts.
Harding EF +4 more
europepmc +3 more sources
Importin-β Family Members Mediate Alpharetrovirus Gag Nuclear Entry via Interactions with Matrix and Nucleocapsid [PDF]
Journal of Virology, 2006 ABSTRACT The retroviral Gag polyprotein orchestrates the assembly and release of virus particles from infected cells. We previously reported that nuclear transport of the Rous sarcoma virus (RSV) Gag protein is intrinsic to the virus assembly pathway.
Lisa Z Scheifele +2 more
exaly +3 more sources
Protease-mediated maturation of M-PMV reverse transcriptase into a functional heterodimer. [PDF]
Protein SciAbstract Reverse transcriptase (RT) of retroviruses orchestrates viral replication, yet its structural diversity remains poorly understood. Well‐studied RTs, such as those from HIV‐1, murine leukemia virus, and avian myeloblastosis virus, were characterized decades ago, but less prominent retroviruses have escaped detailed analysis.
Kapisheva M +6 more
europepmc +2 more sources
Gene Therapy for Primary Immunodeficiency [PDF]
HemaSphere, Volume 5, Issue 1, January 2021., 2021 Over the past 3 decades, there has been significant progress in refining gene therapy technologies and procedures. Transduction of hematopoietic stem cells ex vivo using lentiviral vectors can now create a highly effective therapeutic product, capable of
Booth, C, Houghton, BC
core +2 more sources
Virology, 2006 The Rous sarcoma virus (RSV) Gag polyprotein is the only protein required for virus assembly and release. We previously found that deletion of either one of the two Cys-His (CH) motifs in the RSV nucleocapsid (NC) protein did not abrogate Gag-Gag interactions, RNA binding, or packaging but greatly reduced virus production (E-G. Lee, A.
Eun-Gyung Lee, Maxine L Linial
exaly +3 more sources
How HIV-1 Uses the Metabolite Inositol Hexakisphosphate to Build Its Capsid. [PDF]
VirusesThe HIV-1 capsid is one of virology's most iconic structures, yet how it assembles has long remained elusive. Remarkably, the capsid is made from just a single protein, CA, which forms a lattice of ~250 hexamers and exactly 12 pentamers.
James LC.
europepmc +3 more sources