Results 41 to 50 of about 117,696 (250)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Gly Betaine Surpasses Melatonin to Improve Salt Tolerance in Dalbergia odorifera

Frontiers in Plant Science, 2021
Salinity is one of the most serious factors limiting plant growth which can provoke significant losses in agricultural crop production, particularly in arid and semi-arid areas.
El Hadji Malick Cisse, Ling-Feng Miao, Fan Yang, Jin-Fu Huang, Da-Dong Li, Juan Zhang   +5 more
doaj   +1 more source

Structure of the trypanosome cyanide-insensitive alternative oxidase [PDF]

Proceedings of the National Academy of Sciences, 2013
In addition to haem copper oxidases, all higher plants, some algae, yeasts, molds, metazoans, and pathogenic microorganisms such as Trypanosoma brucei contain an additional terminal oxidase, the cyanide-insensitive alternative oxidase (AOX).
Tomoo, Shiba, Yasutoshi, Kido, Kimitoshi, Sakamoto, Daniel Ken, Inaoka, Chiaki, Tsuge, Ryoko, Tatsumi, Gen, Takahashi, Emmanuel Oluwadare, Balogun, Takeshi, Nara, Takashi, Aoki, Teruki, Honma, Akiko, Tanaka, Masayuki, Inoue, Shigeru, Matsuoka, Hiroyuki, Saimoto, Anthony L, Moore, Shigeharu, Harada, Kiyoshi, Kita   +17 more
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Ascorbate synthesis as an alternative electron source for mitochondrial respiration: Possible implications for the plant performance

Frontiers in Plant Science, 2022
The molecule vitamin C, in the chemical form of ascorbic acid (AsA), is known to be essential for the metabolism of humans and animals. Humans do not produce AsA, so they depend on plants as a source of vitamin C for their food. The AsA synthesis pathway
Isabelle Faria Matos, Luis Miguel Mazorra Morales, Diederson Bortolini Santana, Gláucia Michelle Cosme Silva, Mara Menezes de Assis Gomes, Ricardo Antônio Ayub, José Hélio Costa, José Hélio Costa, Jurandi Gonçalves de Oliveira   +8 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Alternative oxidase in the branched mitochondrial respiratory network: an overview on structure, function, regulation, and role

Brazilian Journal of Medical and Biological Research, 1998
Plants and some other organisms including protists possess a complex branched respiratory network in their mitochondria. Some pathways of this network are not energy-conserving and allow sites of energy conservation to be bypassed, leading to a decrease ...
Sluse F.E., Jarmuszkiewicz W.
doaj  

‘Breathing Out’ under Heat Stress—Respiratory Control of Crop Yield under High Temperature

Agronomy, 2022
Respiration and photosynthesis are indispensable plant metabolic processes that are affected by elevated temperatures leading to disruption of the carbon economy of the plants.
Nitin Sharma, Meenakshi Thakur, Pavithra Suryakumar, Purbali Mukherjee, Ali Raza, Channapatna S. Prakash, Anjali Anand   +6 more
doaj   +1 more source

Analysis of Posttranslational Activation of Alternative Oxidase Isoforms [PDF]

Plant Physiology, 2017
Mitochondrial alternative oxidase (AOX) in plants is a non-proton-motive ubiquinol oxidase that is activated by redox mechanisms and 2-oxo acids. A comparative analysis of the AOX isoenzymes AOX1A, AOX1C, and AOX1D from Arabidopsis (Arabidopsis thaliana) revealed that cysteine residues, CysI and CysII, are both involved in 2-oxo acid activation, with ...
Jennifer Selinski, Andreas Hartmann, Adrian Kordes, Gabriele Deckers-Hebestreit, James Whelan, Renate Scheibe   +5 more
openaire   +2 more sources