Results 271 to 280 of about 218,037 (308)
Some of the next articles are maybe not open access.
Current Gene Therapy, 2006
HSV-1 amplicon vectors efficiently transduce cultured antigen-presenting cells (APC), including both human and murine dendritic cells as well as primary human chronic lymphocytic leukemia (CLL) B cells. Helper-free amplicons have been shown to be especially well-suited for this purpose, since they do not impair the antigen-presenting functions of these
Kathlyn, Santos +2 more
openaire +2 more sources
HSV-1 amplicon vectors efficiently transduce cultured antigen-presenting cells (APC), including both human and murine dendritic cells as well as primary human chronic lymphocytic leukemia (CLL) B cells. Helper-free amplicons have been shown to be especially well-suited for this purpose, since they do not impair the antigen-presenting functions of these
Kathlyn, Santos +2 more
openaire +2 more sources
Quantifying Amplicons with ELISA
2003Among the numerous assays proposed for quantifying specific nucleic-acid sequences in biological samples, PCR offers the greatest sensitivity and versatility. The assay for quantifying the amount of polymerase chain reaction (PCR) products is a crucial step in any quantitative PCR method.
O, Lantz, E, Bonney, Y, Taoufik
openaire +2 more sources
The 17q23 Amplicon and Breast Cancer
Breast Cancer Research and Treatment, 2003A novel region of amplification in breast tumors was recently identified on chromosome 17q23. Extensive mapping of the amplicon by Southern blotting and fluorescence in situ hybridization (FISH) in breast cancer cell lines determined that the amplicon can be up to 4 Mbp in size and may contain 50 genes.
Colleen S, Sinclair +3 more
openaire +2 more sources
Targeting HSV amplicon vectors
Methods, 2004Several techniques have been developed to deliver DNA directly into mammalian cells, spanning in difficulty from simple mixing procedures to complex systems requiring expensive equipment. Viral vectors have proven able to deliver genes into mammalian cells with high efficiency and low toxicity.
GRANDI, Paola +3 more
openaire +2 more sources
HSV Amplicons: Neuro Applications
Current Gene Therapy, 2006Strategies that employ HSV amplicon vectors in the prevention and/or amelioration of pathogenic states afflicting the central nervous system (CNS) have been extensively documented in preclinical disease models. The versatility of the HSV amplicon platform allows for the implementation of therapeutic approaches that require expression of genes ...
Carolyn M, Tyler +3 more
openaire +2 more sources
Amplicon Sequencing Pipelines in Metagenomics
2023Taxonomic profiling among a large number of samples is a fundamental task during amplicon sequencing analysis. The heterogeneity and technical noises in the sample handling, library preparation, and sequencing present a major challenge to how the biological conclusions are drawn from the data analysis, and accordingly, many tools have been developed to
openaire +2 more sources
2003
Herpes simplex virus (HSV) amplicons are defective virus vectors capable of introducing amplified foreign genes into variable types of eukaryotic cells, such as fibroblasts, macrophages, glia, and neurons in different organisms including rodents, monkeys, and human (refs. 1-3; reviewed in ref. 4).
N, Frenkel, R, Sarid
openaire +2 more sources
Herpes simplex virus (HSV) amplicons are defective virus vectors capable of introducing amplified foreign genes into variable types of eukaryotic cells, such as fibroblasts, macrophages, glia, and neurons in different organisms including rodents, monkeys, and human (refs. 1-3; reviewed in ref. 4).
N, Frenkel, R, Sarid
openaire +2 more sources
HSV Amplicon Vectors for Cancer Therapy
Current Gene Therapy, 2006HSV amplicon vectors provide a unique tool in the armamentarium of weapons for treatment of cancer. Their large capacity (up to 150 kb) allows incorporation of multiple and large transgenes, including whole gene loci, as well as components of other viruses to control the fate of transgenes in the host cells.
Khalid, Shah, Xandra O, Breakefield
openaire +2 more sources
1998
Almost two centuries ago, long before they were identified as infectious agents, viruses were used for therapeutic applications. In 1798, Jenner discovered that persons inoculated with less pathogenic cowpox (cowpox virus) were protected from subsequent infection with deadly smallpox (variola virus) (1).
Cornel Fraefel +2 more
openaire +1 more source
Almost two centuries ago, long before they were identified as infectious agents, viruses were used for therapeutic applications. In 1798, Jenner discovered that persons inoculated with less pathogenic cowpox (cowpox virus) were protected from subsequent infection with deadly smallpox (variola virus) (1).
Cornel Fraefel +2 more
openaire +1 more source
Current Gene Therapy, 2006
We have derived the HSV amplicon vector in 1981/1982 after elaborate experience with "defective viruses", arising spontaneously in viral stocks propagated at high multiplicities of infection (m.o.i.). The defective viruses were found to contain large concatemeric genomes with repeat units of limited complexity.
openaire +2 more sources
We have derived the HSV amplicon vector in 1981/1982 after elaborate experience with "defective viruses", arising spontaneously in viral stocks propagated at high multiplicities of infection (m.o.i.). The defective viruses were found to contain large concatemeric genomes with repeat units of limited complexity.
openaire +2 more sources