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Targeted Amplicon Genotyping by Sanger Sequencing
This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated ...Anthony Torres, Reginald Gaudino
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Microbial diagnosis of endophthalmitis using nanopore amplicon sequencing
International Journal of Medical Microbiology, 2021Kang Il Jun, Baek-Lok Oh, Narae Kim
exaly
A critical perspective on interpreting amplicon sequencing data in soil ecological research
Soil Biology and Biochemistry, 2021Lauren V Alteio +2 more
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Phylogenetic Placement of Exact Amplicon Sequences Improves Associations with Clinical Information
MSystems, 2018Stefan Janssen +2 more
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