Results 171 to 180 of about 860,088 (184)
Some of the next articles are maybe not open access.
Amplicon Sequencing using the Long-Read Sequencing Technologies
Journal of Visualized ExperimentsThe World Health Organization (WHO) continues to emphasize the urgent need for a rapid, cost-effective, and user-friendly diagnostic method for tuberculosis (TB) and drug-resistant TB (DR-TB). Next-generation sequencing (NGS) technologies, endorsed by the WHO, have significantly improved the detection of DR-TB.
Morwasehla, Modjadji +7 more
openaire +2 more sources
Targeted Amplicon Genotyping by Sanger Sequencing
This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated ...Anthony Torres, Reginald Gaudino
openaire +2 more sources
16S rRNA Amplicon Sequencing for Metagenomics
2018The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The results can be used to evaluate microbial diversity at genus, family, order, class, and phylum levels. The resolution is normally insufficient to evaluate the species level.
Henrik Christensen +3 more
openaire +1 more source
Effect of Amplicon Sequencing Depth in Environmental Microbiome Research
Current Microbiology, 2021Amplicon sequencing approach is commonly employed in microbiome studies and sequencing depth is considered as a major factor influencing the outcome of data analyses. As of now, the effect of amplicon sequencing depth in environmental microbiome analyses is not explicitly illustrated.
openaire +2 more sources
Species Typing of Trypanosomatids Using Amplicon-Based Sequencing
Amplicon-based sequencing has emerged as an exceptionally powerful tool for the identification and characterization of microorganisms, offering enhanced sensitivity and specificity in molecular diagnostics. In this chapter, we detail the amplification and sequencing protocols for two critical genetic markers: the HSP70 gene, essential for identifying ...Cristian, Canizales-Silva +2 more
openaire +2 more sources
Automatic identification of contaminating sequences in amplicon sequencing data
The Thirteenth International Multiconference, 2022openaire +1 more source
Amplicon preparation and sequencing on solid supports
2015XU HONGXIA, ARAVANIS ALEX, LIN SHENGRONG
openaire +3 more sources