Targeted amplicon sequencing for enhanced detection of spiked Shiga toxin-producing <i>Escherichia coli</i> in ready-to-eat romaine lettuce: a proof-of-concept study. [PDF]
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Comparative 16S rRNA Gene Amplicon Sequencing of the Fecal Microbiome in Pet Dogs and Cats of Different Breeds in Dhaka City, Bangladesh: With Preliminary Insights Into Zoonotic Relevance. [PDF]
Tania MNT +9 more
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Gut and fecal microbiome profiles of the electric eel (<i>Electrophorus varii</i>) revealed by 16S rRNA amplicon sequencing. [PDF]
Hong J +5 more
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Rapid bacterial community profiling of equine faecal, skin, milk and saliva samples using Oxford Nanopore long-read 16S rRNA amplicon sequencing. [PDF]
Leng J +6 more
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Target gene mutation discovery and amplicon sequencing for evaluating maduramicin resistance in Eimeria tenella. [PDF]
Gao Y +10 more
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Amplicon Sequencing Pipelines in Metagenomics
2023Taxonomic profiling among a large number of samples is a fundamental task during amplicon sequencing analysis. The heterogeneity and technical noises in the sample handling, library preparation, and sequencing present a major challenge to how the biological conclusions are drawn from the data analysis, and accordingly, many tools have been developed to
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Amplicon sequence data of locust
2022Changes of microbial community in migratory locusts after Beauveria bassiana infection.
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Practical innovations for high-throughput amplicon sequencing
Nature Methods, 2013We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide.
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