Results 51 to 60 of about 25,156 (264)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Teaching Life Sciences to English second language learners: what do teachers do?

South African Journal of Education, 2011
South Africa has eleven official languages and legally learners receive tuition in their mother tongue until the end of Grade 3. From then on teachers are required to teach through the medium of English or Afrikaans.
Johanna G Ferreira
doaj   +2 more sources

Mihailo Petrović's contributions to the philosophy of phenomenological ideas (compared to their most famous representative, Edmund Husserl) [PDF]

Baština
The aim of this paper is to present phenomenology as a direction of contemporary philosophy, whose most famous representative is Edmund Husserl (Edmund Husserl, 1859-1938), from the perspective of the mathematical phenomenology of Mihailo Petrović (1868 ...
Mijailović Vladana Z.
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Discursive interactions and the use of analogies in physics teaching [PDF]

Investigações em Ensino de Ciências, 2012
In the last few years, Science Education researchers have shown new ways do conceive the processes of teaching and learning, demanding the displacement from the individual understanding of specific phenomena to a new context, which means, to a bias of ...
Fernanda Cátia Bozelli, Roberto Nardi
doaj  

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Conceptual change, subjectivity and Psychoanalysis

Investigações em Ensino de Ciências, 1997
We present the Conceptual Change Model (Posner et al., 1982) and detach how the presence of student’s subjectivity deeply marks the four conditions for the accomodation. The fundamental metaphor which supports the model, student as a little scientist, is
Alberto Villani, Tânia Cristina Baptista Cabral   +1 more
doaj  

Impact of Analogies and Metaphors in Propagating Misconceptions in Chemical Education

Shodh Sari
Analogies and metaphors have been used as teaching tools in chemical education to make complex concepts easier to understand. While they help students grasp ideas at first, these language and visual aids can also create lasting misconceptions that make ...
Dr Sandeep Kumar
doaj   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Early Clinical, Imaging, and Pathological Characteristics of SRPK3/TTN‐Digenic Myopathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective SRPK3/TTN‐digenic myopathy was recently established as a skeletal muscle myopathy caused by digenic inheritance. This study characterizes the early clinical presentation of SRPK3/TTN‐digenic myopathy in one previously reported and seven newly identified pediatric patients.
Rotem Orbach, Sandra Donkervoort, Carola Hedberg‐Oldfors, Giovanni Baranello, Dimah Saade, Precilla D'Souza, Ruchee Patel, Eva Michael, A. Reghan Foley, Diana Bharucha‐Goebel, S. Jin Haugland, Meghan McAnally, Omer Abdul Hamid, Katherine Chao, Ellen F. Macnamara, Alan H. Beggs, Anna Sarkozy, Juliane Mueller, Steven A. Moore, Richard S. Finkel, Cynthia J. Tifft, Francesco Muntoni, Anders Oldfors, Carsten G. Bönnemann   +23 more
wiley   +1 more source