Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Typological Parallels and Differences in the Poetry of Taras Shevchenko and Slovak Romantic Poets (Samo Chalupka, Andrej Sládkovič, Janko Kráľ, Ján Botto) [PDF]
The paper aims at identifying possible typological overlaps between the works of Slovak romantic poets (Samo Chalupka, Andrej Sládkovič, Janko Kráľ, Ján Botto) and the most prominent Ukrainian writer Taras Shevchenko.
Oleksandr Boroň
doaj
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
This article examines innovative approaches to augmenting science lessons taught in middle and high school, with special emphasis on theimportance of the early teen years, when experiences both in and out of school have significant impact on career ...
Thomas McNally
doaj +2 more sources
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Analogies as a potential for expressing creativity at different levels [PDF]
People living in the modern world face an increasing number of serious problems, the solution to which requires a creative approach. Therefore, the issue of creativity development and the factors that influence it are becoming increasingly important, as ...
Cvjetinović Vesna Z. +1 more
doaj +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Items of Horse Equipment From Zuevsky Burial Ground of Ananyinо Culture of Cord Ceramics in Udmurtia
The paper has been prepared in connection with the 120th anniversary of the Zuevsky burial ground. Zuevsky (Zuevy Kliuchi I) burial ground is one of the few large Ananyino necropolises in the cis-Kama region, which items are used in cultural and ...
Chernykh Elizaveta M.
doaj +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source

