Results 111 to 120 of about 8,447,464 (395)
Laboratory Animals. Journal of the Laboratory Animal Science Association, 2019 The European Federation of Pharmaceutical Industries and Associations’ Research and Animal Welfare group members reflected on the concept of a Culture of Care in relation to animal care and use and on differences in its understanding and application ...
Sally Robinson +6 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield +3 more
wiley +1 more source
Frontiers in Microbiology, 2019 Growth retardation reduces the incomes of livestock farming. However, effective nutritional interventions to promote compensatory growth and the mechanisms involving digestive tract microbiomes and transcripts have yet to be elucidated.
Rui Hu +14 more
doaj +1 more source
In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
, 2010 We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada +9 more
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Science, Culture, and Care in Laboratory Animal Research
, 2018 The principles of the 3Rs—replacement, refinement, and reduction—strongly shape discussion of methods for performing more humane animal research and the regulation of this contested area of technoscience.
G. Davies +3 more
semanticscholar +1 more source
A Simple Method to Reduce both Lactic Acid and Ammonium Production in Industrial Animal Cell Culture
International Journal of Molecular Sciences, 2018 Fed-batch animal cell culture is the most common method for commercial production of recombinant proteins. However, higher cell densities in these platforms are still limited due to factors such as excessive ammonium production, lactic acid production ...
Nathaniel W. Freund, M. Croughan
semanticscholar +1 more source
FEBS Letters, EarlyView.In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker +8 more
wiley +1 more source
AnimalsThe objectives of the current study were to evaluate the fluctuations in production performance, rumen fermentation, and microbial community in lactating dairy cows fed a high-grain diet (HG).
Kexin Wang +5 more
doaj +1 more source
Italian Journal of Animal Science, 2015 The objective of the experiment was to evaluate the effects of dietary carbohydrate composition on rumen fermentation characteristics and microbial populations in vitro. The treatments were organized in three different carbohydrate composition diets: the
Xiangfei Zhang +6 more
doaj +1 more source
Aquaculture Asia, Vol. 11, No. 1, pp.1-36, January - March 2006 [PDF]
, 2006 *Table of Contents* Research & farming techniques Nursery rearing of Puntius goniotus: A preliminary trial K.N. Mohnta, J.K. Jena & S.N. Mohanty Artemia enrichment and biomass production for larval finfish and shellfish culture A.S.
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