Results 191 to 200 of about 1,351,389 (261)

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Decoding Sebaceous Gland Biology Through Experimental Models: Progress, Limitations, and Future Directions. [PDF]

Cells
Briganti S, Mosca S, Ottaviani M, Flori E.   +3 more
europepmc   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Multi-dimensional dynamic assessment revealed the antibiotic-induced pseudo-germ-free model in SD rats stabilized within 4 days. [PDF]

Front Microbiol
Zhao Y, Wang L, Zhang K, Zhang J, Guo Z, Lu X, Li J.   +6 more
europepmc   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing. [PDF]

J Transl Med
Caicedo A, Benavides-Almeida A, Peñaherrera S, Robayo P, Villagómez A, León M, Suárez-Usbeck A, Padilla-Sánchez SD, Santacruz M, Borja T, Arteaga MB, Haro-Vinueza A, Segnini G, Pontón P, Torres F, Donoso G, Suquillo D, Dos Santos LF, Arizo P, Tenesaca D, Antilef B, Zavala G, Barba D, Ferrada L, Del Campo A, Zambrano K, Chile-Miranda S, Viera-Catota C, Cisterna S, Villavicencio D, Pérez-Meza ÁA, Cisneros-Heredia DF, Aponte PM, Cabrera F, Luz-Crawford P, Moya IM, Mitrani MI, Khoury M, Nova-Lamperti E, Burzio VA, Maron-Gutierrez T, Díaz RF.   +41 more
europepmc   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Integrated Field Evaluation of a Caseous Lymphadenitis Vaccine and Environmental Bacteriological Profiling in Commercial Goat Farms in South Korea. [PDF]

Vaccines (Basel)
Park GS, Lee S, Park M, Kim M, Lee E, Lee S, Kim MH, Jung BY, Kim C, Seo BJ.   +9 more
europepmc   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

A vascular calcification model enabled by an <i>ex vivo</i> vascular calcification perfusion system. [PDF]

iScience
Li J, Gao Z, Wang B, Wang S, Sun M, Tian Y, Ma T.   +6 more
europepmc   +1 more source